Western Blot (WB)
(CHD2 pAb tested by Western blot. Detection of CHD2 by Western blot analysis. Nuclear extract of HEK293 cells (25 ug) was probed with CHD2 pAb at a 1:250 dilution.)
Rabbit CHD2 Polyclonal Antibody | anti-CHD2 antibody
CHD2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot
Purity
Affinity Purified
Synonyms
CHD2; Polyclonal Antibody; CHD2 antibody (pAb); EEOC; anti-CHD2 antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-CHD2 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:250-1:1,000 dilution
The addition of 0.1% Tween 20 in all blocking solutions may reduce background.
WB: 1:250-1:1,000 dilution
The addition of 0.1% Tween 20 in all blocking solutions may reduce background.
Immunogen
This CHD2 antibody (pAb) was raised against a peptide corresponding to the C-terminus of human CHD2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(CHD2 pAb tested by Western blot. Detection of CHD2 by Western blot analysis. Nuclear extract of HEK293 cells (25 ug) was probed with CHD2 pAb at a 1:250 dilution.)
Western Blot (WB)
(CHD2 pAb tested by Western blot. Detection of CHD2 by Western blot analysis. Nuclear extract of HEK293 cells (25 ug) was probed with CHD2 pAb at a 1:250 dilution.)
Related Product Information for anti-CHD2 antibody
Short Description: Our CHD2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 is required for normal development of mouse embryos.
Background: CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 is required for normal development of mouse embryos.
Product Categories/Family for anti-CHD2 antibody
NCBI and Uniprot Product Information
Molecular Weight
260kDa
Protein Family
Similar Products
Product Notes
The CHD2 (Catalog #AAA388457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHD2 antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's CHD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:250-1:1,000 dilution The addition of 0.1% Tween 20 in all blocking solutions may reduce background. Researchers should empirically determine the suitability of the CHD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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