Western Blot (WB)
(CARM1 pAb tested by Western blot. Whole-cell extract of HEK293 cells (10 ug) probed with CARM1 pAb at a dilution of 1:500.)
Rabbit CARM1 Polyclonal Antibody | anti-CARM1 antibody
CARM1 antibody (pAb)
WB: 1:250-1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Western Blot (WB)
(CARM1 pAb tested by Western blot. Whole-cell extract of HEK293 cells (10 ug) probed with CARM1 pAb at a dilution of 1:500.)
Western Blot (WB)
(CARM1 pAb tested by Western blot. Whole-cell extract of HEK293 cells (10 ug) probed with CARM1 pAb at a dilution of 1:500.)
Background: CARM1 (Coactivator-associated arginine methyltransferase 1 or CARM 1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. CARM1 methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.
NCBI and Uniprot Product Information
Uniprot Description
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
Research Articles on CARM1
Similar Products
Product Notes
The CARM1 carm1 (Catalog #AAA388430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARM1 antibody (pAb) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CARM1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 1:250-1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the CARM1 carm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
