Chromatin Immunoprecipitation (ChIP)
(PBRM1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse liver and 4 ug PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites.)
Rabbit anti-Human, Mouse PBRM1 Polyclonal Antibody | anti-PBRM1 antibody
PBRM1 antibody (pAb)
Gene Names
PBRM1; PB1; BAF180
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
PBRM1; Polyclonal Antibody; PBRM1 antibody (pAb); PB1; BAF180; anti-PBRM1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-PBRM1 antibody
Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB)
Application Notes
Applications Validated:
ChIP-Seq: 7ul (4 ug) per ChIP
WB: 1:500-1:1,000 dilution
Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
ChIP-Seq: 7ul (4 ug) per ChIP
WB: 1:500-1:1,000 dilution
Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human PBRM1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(PBRM1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse liver and 4 ug PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites.)
Chromatin Immunoprecipitation (ChIP)
(PBRM1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse liver and 4 ug PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites.)
Western Blot (WB)
(PBRM1 antibody (pAb) tested by Western blot. Detection of PBRM1 by Western blot. The analysis was performed using 40 ug of A-431 nuclear extract and PBRM1 (pAb) at a 1:1000 dilution.)
Western Blot (WB)
(PBRM1 antibody (pAb) tested by Western blot. Detection of PBRM1 by Western blot. The analysis was performed using 40 ug of A-431 nuclear extract and PBRM1 (pAb) at a 1:1000 dilution.)
Related Product Information for anti-PBRM1 antibody
Short Description: Our PBRM1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq and Western blot; it has been shown to react with Human and Mouse samples.
Background: PBRM1 (polybromo 1) is a component of the SWI/SNF-B (PBAF) ATP-dependent chromatin-remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling and is an integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Defects in PBRM1 have been associated with primary clear cell renal cell carcinoma.
Background: PBRM1 (polybromo 1) is a component of the SWI/SNF-B (PBAF) ATP-dependent chromatin-remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling and is an integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Defects in PBRM1 have been associated with primary clear cell renal cell carcinoma.
Product Categories/Family for anti-PBRM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
181kDa
NCBI Official Full Name
protein polybromo-1 isoform 2
NCBI Official Synonym Full Names
polybromo 1
NCBI Official Symbol
PBRM1
NCBI Official Synonym Symbols
PB1; BAF180
NCBI Protein Information
protein polybromo-1
UniProt Protein Name
Protein polybromo-1
Protein Family
UniProt Gene Name
PBRM1
UniProt Synonym Gene Names
BAF180; PB1; hPB1; BAF180
UniProt Entry Name
PB1_HUMAN
NCBI Description
This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012]
Research Articles on PBRM1
Similar Products
Product Notes
The PBRM1 pbrm1 (Catalog #AAA388870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PBRM1 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PBRM1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP-Seq: 7ul (4 ug) per ChIP WB: 1:500-1:1,000 dilution Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the PBRM1 pbrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBRM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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