Mouse anti-Human SaCas9 Antibody | anti-SaCas9 antibody

AbFlex SaCas9 antibody (rAb)

Cat. Num. AAA389497

• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A Chromatography
• Synonyms: SaCas9; AbFlex SaCas9 antibody (rAb); CRISPR/Cas9; anti-SaCas9 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Purity/Purification: Protein A Chromatography
• Form/Format: Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-SaCas9 antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 0.5-2ug/ml; AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
• Antibody Type: Recombinant Antibody
• Immunogen: This antibody was raised against a recombinant protein fragment containing amino acids 1-462 of Staphylococcus aureus Cas9.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

SDS-Page

(AbFlex SaCas9 antibody (rAb) tested by Western blot. 20 ug of HeLa whole-cell extract (Lane 1) and HeLa whole-cell extract with 10 ng of full length SaCas9 protein (Lane 2), was run on SDS-PAGE and probed with AbFlex SaCas9 antibody at 0.5 ug/ml.)

Related Product Information for anti-SaCas9 antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex SaCas9 antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex SaCas9 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. SaCas9 is a nuclease from Staphylococcus aureus that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome.

Product Categories/Family for anti-SaCas9 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• UniProt Accession #: J7RUA5
• Molecular Weight: 130kDa
• UniProt Protein Name: CRISPR-associated endonuclease Cas9
• UniProt Gene Name: cas9

Uniprot Description

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.