Rabbit anti-Human Rrn3 Polyclonal Antibody | anti-Rrn3 antibody

Rrn3 (phospho Ser649) polyclonal antibody

Cat. Num. AAA3002844

• Gene Names: RRN3; TIFIA; A-270G1.2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Rrn3; Polyclonal Antibody; Rrn3 (phospho Ser649) polyclonal antibody; RRN3; TIFIA; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA; anti-Rrn3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: RRN3; TIFIA; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-Rrn3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/10,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human TIF-IA around the phosphorylation site of Ser649. AA range: 602-651
• Function: Required for efficient transcription initiation by RNA polymerase I.
• Similarity: Belongs to the RRN3 family.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 93102377
• NCBI GeneID: 54700
• NCBI Accession #: NP_060897.3
• NCBI GenBank Nucleotide #: NM_018427.3
• UniProt Accession #: Q9NYV6; Q6IPL4; Q9H4F0; A2RTY9
• Molecular Weight: 74,107 Da
• NCBI Official Full Name: RNA polymerase I-specific transcription initiation factor RRN3
• NCBI Official Synonym Full Names: RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
• NCBI Official Symbol: RRN3
• NCBI Official Synonym Symbols: TIFIA; A-270G1.2
• NCBI Protein Information: RNA polymerase I-specific transcription initiation factor RRN3; TIF-IA; transcription initiation factor IA; transcription initiation factor TIF-IA
• UniProt Protein Name: RNA polymerase I-specific transcription initiation factor RRN3
• Protein Family: RNA polymerase I-specific transcription initiation factor
• UniProt Gene Name: RRN3
• UniProt Synonym Gene Names: TIFIA; TIF-IA
• UniProt Entry Name: RRN3_HUMAN

Uniprot Description

TIF1A: a transcription initiation factor. RNA polymerase I transcription factor RRN3. A growth-dependent regulator of ribosomal RNA synthesis

Protein type: Transcription initiation complex; Nucleolus

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: nucleoplasm; nucleolus; nucleus

Biological Process: cell proliferation; in utero embryonic development; positive regulation of transcription, DNA-dependent; ribosome biogenesis and assembly; homeostasis of number of cells; transcription from RNA polymerase I promoter; gene expression; cytoplasm organization and biogenesis; transcription initiation from RNA polymerase I promoter; nucleolus organization and biogenesis