SDS-Page
(AbFlex Mi-2 beta (CHD4) antibody (rAb) tested by Western blot 20 ug of Jurkat cell nuclear extract was run on SDS-PAGE and probed with AbFlex Mi-2 beta (CHD4) antibody at 0.5 ug/ml.)
Mouse anti-Human Mi-2 beta Antibody | anti-Mi-2beta antibody
AbFlex Mi-2 beta (CHD4) antibody (rAb)
Gene Names
CHD4; CHD-4; Mi-2b; Mi2-BETA
Reactivity
Human
Applications
Western Blot
Purity
Protein A Chromatography
Synonyms
Mi-2 beta; AbFlex Mi-2 beta (CHD4) antibody (rAb); CHD4; Mi-2b; anti-Mi-2beta antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Mi-2beta antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml
AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
WB: 0.5-2ug/ml
AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a synthetic peptide derived from human Mi-2 beta.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex Mi-2 beta (CHD4) antibody (rAb) tested by Western blot 20 ug of Jurkat cell nuclear extract was run on SDS-PAGE and probed with AbFlex Mi-2 beta (CHD4) antibody at 0.5 ug/ml.)
SDS-Page
(AbFlex Mi-2 beta (CHD4) antibody (rAb) tested by Western blot 20 ug of Jurkat cell nuclear extract was run on SDS-PAGE and probed with AbFlex Mi-2 beta (CHD4) antibody at 0.5 ug/ml.)
Related Product Information for anti-Mi-2beta antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Mi-2 beta (CHD4) antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Mi-2 beta (CHD4) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Mi-2b (Mi-2 beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Mi-2 beta (CHD4) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Mi-2b (Mi-2 beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).
Product Categories/Family for anti-Mi-2beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
220,848 Da
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 4 isoform 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 4
NCBI Official Symbol
CHD4
NCBI Official Synonym Symbols
CHD-4; Mi-2b; Mi2-BETA
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 4
UniProt Gene Name
CHD4
UniProt Synonym Gene Names
CHD-4
UniProt Entry Name
CHD4_HUMAN
Uniprot Description
CHD-4: is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells.
Protein type: Helicase; DNA-binding; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: nucleoplasm; centrosome; protein complex; membrane; nuclear chromatin; cytoplasm; NuRD complex; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding; nucleosomal DNA binding; DNA binding; zinc ion binding; microtubule binding; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; ATP-dependent chromatin remodeling; spindle assembly; DNA duplex unwinding
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Product Notes
The Mi-2beta chd4 (Catalog #AAA389495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Mi-2 beta (CHD4) antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mi-2 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Mi-2beta chd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mi-2 beta, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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