SDS-Page
(AbFlex Pim2 antibody (rAb) tested by Western blot. 20 ug of K562 cytoplasmic extract was run on SDS-PAGE and probed with AbFlex Pim2 antibody at 0.5 ug/ml. This antibody recognizes all three isoforms of human Pim2.)
Mouse anti-Human Pim2 Antibody | anti-Pim2 antibody
AbFlex Pim2 antibody (rAb)
WB: 0.5-2ug/ml
Note: For optimal results, we recommend the addition of 0.1% Tween to all blocking solutions to reduce background. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
SDS-Page
(AbFlex Pim2 antibody (rAb) tested by Western blot. 20 ug of K562 cytoplasmic extract was run on SDS-PAGE and probed with AbFlex Pim2 antibody at 0.5 ug/ml. This antibody recognizes all three isoforms of human Pim2.)
SDS-Page
(AbFlex Pim2 antibody (rAb) tested by Western blot. 20 ug of K562 cytoplasmic extract was run on SDS-PAGE and probed with AbFlex Pim2 antibody at 0.5 ug/ml. This antibody recognizes all three isoforms of human Pim2.)
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Pim2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody recognizes all three isoforms of human Pim2. PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-kB kinase/NF-kB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.[provided by RefSeq, Nov 2009]
Uniprot Description
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Research Articles on Pim2
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Product Notes
The Pim2 pim2 (Catalog #AAA389478) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Pim2 antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pim2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml Note: For optimal results, we recommend the addition of 0.1% Tween to all blocking solutions to reduce background. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Pim2 pim2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pim2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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