SDS-Page
(AbFlex Ubiquitin antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear acid-extract was run on SDS-PAGE and probed with AbFlex Ubiquitin antibody at 0.5 ug/ml. Antibodies to Ubiquitin will detect proteins of various sizes that are modified by the addition of Ubiquitin.)
Mouse Ubiquitin Antibody
AbFlex Ubiquitin antibody (rAb)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot
Purity
Protein A Chromatography
Synonyms
Ubiquitin; AbFlex Ubiquitin antibody (rAb); anti-Ubiquitin antibody
Host
Mouse
Reactivity
Human, Wide Range Predicted
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Ubiquitin antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml
DB: 2ug/ml
AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
WB: 0.5-2ug/ml
DB: 2ug/ml
AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against bovine ubiquitin.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex Ubiquitin antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear acid-extract was run on SDS-PAGE and probed with AbFlex Ubiquitin antibody at 0.5 ug/ml. Antibodies to Ubiquitin will detect proteins of various sizes that are modified by the addition of Ubiquitin.)
SDS-Page
(AbFlex Ubiquitin antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear acid-extract was run on SDS-PAGE and probed with AbFlex Ubiquitin antibody at 0.5 ug/ml. Antibodies to Ubiquitin will detect proteins of various sizes that are modified by the addition of Ubiquitin.)
Dot Blot (DB)
(AbFlex Ubiquitin antibody specifity Dot blot analysis was used to confirm the specificity of Ubiquitin antibody. 50 ng, 10 ng, and 2 ng of either recombinant Ubiquitin (Lane 1) or BSA (Lane 2) were spotted onto nylon membrane and probed with AbFlex Ubiquitin recombinant antibody at 2 ug/ml.)
Dot Blot (DB)
(AbFlex Ubiquitin antibody specifity Dot blot analysis was used to confirm the specificity of Ubiquitin antibody. 50 ng, 10 ng, and 2 ng of either recombinant Ubiquitin (Lane 1) or BSA (Lane 2) were spotted onto nylon membrane and probed with AbFlex Ubiquitin recombinant antibody at 2 ug/ml.)
Related Product Information for anti-Ubiquitin antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Ubiquitin antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ubiquitin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ubiquitin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.
Product Categories/Family for anti-Ubiquitin antibody
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The Ubiquitin (Catalog #AAA389486) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Ubiquitin antibody (rAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml DB: 2ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Ubiquitin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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