Typical Testing Data/Standard Curve (for reference only)
(Mouse CD23/FCER2 PicoKine ELISA Kit standard curve )
Mouse CD23/FCER2 PicoKine ELISA Kit | Fcer2a elisa kit
Mouse CD23/FCER2 PicoKine ELISA Kit
Gene Names
Fcer2a; CD23; Fce2; Fcer2; Ly-42
Reactivity
Mouse
Synonyms
CD23/FCER2 PicoKine; Mouse CD23/FCER2 PicoKine ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor; BLAST 2; BLAST-2; C-type lectin domain family 4 member J; CD23; CD23 antigen; CD23A; CLEC4J; Fc fragment of IgE low affinity II receptor for (CD23); Fc-epsilon-RII; FCE2; Fcer2; FCER2_HUMAN; IGEBF; Immunoglobulin E-binding factor; Low affinity immunoglobulin epsilon Fc receptor soluble form; Lymphocyte IgE receptor; Fc fragment of IgE; low affinity II; receptor for (CD23); Fcer2a elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD23
Sequence Length
330
Assay Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Sample
For quantitative detection of mouse CD23 in cell culture supernates, serum and plasma(heparin, EDTA).
Immunogen
NSO, E50-P331
Assay Procedure Step 1
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD23 standard solution and each sample be measured in duplicate.
Intra-Assay Precision
Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|485|1882|8430|597|2347|9105
Standard deviation|24.25|105.40|522.66|42.39|185.41|783.03
CV(%)|5.0|5.6|6.2|7.1|7.9|8.6
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|485|1882|8430|597|2347|9105
Standard deviation|24.25|105.40|522.66|42.39|185.41|783.03
CV(%)|5.0|5.6|6.2|7.1|7.9|8.6
Sample Data
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000
O.D.|0.021|0.045|0.137|0.279|0.537|1.009|1.503|2.141
O.D.|0.021|0.045|0.137|0.279|0.537|1.009|1.503|2.141
Anticoagulant
Heparin or EDTA
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD23/FCER2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD23/FCER2 PicoKine ELISA Kit standard curve )
Related Product Information for Fcer2a elisa kit
Principle of the assay: Mouse CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E50-P331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD23 amount of sample captured in plate.
Background: CD23, also known as Fc epsilon RII, or FcuRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
Background: CD23, also known as Fc epsilon RII, or FcuRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,487 Da
NCBI Official Full Name
low affinity immunoglobulin epsilon Fc receptor isoform B
NCBI Official Synonym Full Names
Fc receptor, IgE, low affinity II, alpha polypeptide
NCBI Official Symbol
Fcer2a
NCBI Official Synonym Symbols
CD23; Fce2; Fcer2; Ly-42
NCBI Protein Information
low affinity immunoglobulin epsilon Fc receptor
UniProt Protein Name
Low affinity immunoglobulin epsilon Fc receptor
UniProt Gene Name
Fcer2
UniProt Synonym Gene Names
Fcer2a
UniProt Entry Name
FCER2_MOUSE
Uniprot Description
FCER2: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Protein type: Membrane protein, integral
Cellular Component: external side of plasma membrane; integral to membrane; membrane; plasma membrane
Molecular Function: carbohydrate binding; IgE binding; metal ion binding
Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of killing of cells of another organism; positive regulation of nitric-oxide synthase activity; positive regulation of nitric-oxide synthase biosynthetic process
Research Articles on Fcer2a
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Product Notes
The Mouse Fcer2a fcer2 (Catalog #AAA177563) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177563 ELISA Kit recognizes Mouse Fcer2a. It is sometimes possible for the material contained within the vial of "CD23/FCER2 PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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