Mouse TFEB Monoclonal Antibody | anti-TFEB antibody

Mouse monoclonal antibody Anti-Human TFEB

Cat. Num. AAA120432

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Applications: Western Blot
• Purity: This antibody was purified using protein A column chromatography from ascites.
• Synonyms: TFEB; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human TFEB; anti-TFEB antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 319C2a
• Purity/Purification: This antibody was purified using protein A column chromatography from ascites.
• Form/Format: Mouse monoclonal anti-human TFEB antibody in PBS (3.0 mM KCl, 1.5 mM KH₂PO₄, 140 mM NaCl, 8.0 mM Na₂HPO₄ (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃).

• Applicable Applications for anti-TFEB antibody: Western Blot (WB)
• Immunogen: Recombinant
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃) as a preservative. There is a potential hazard that NaN₃ reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Safety Warnings and Precautions: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8°C for up to one year.; We recommend storing at -20°C for long-term storage.; Avoid repeat freezing and thawing cycles.

Western Blot (WB)

Immunostaining

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TFEB mAb.)

References

Methods to Monitor and Manipulate TFEB Activity During Autophagy. D.L. Medina, C. Settembre, A. Ballabio (2016)

NCBI and Uniprot Product Information

• NCBI GI #: 268607698
• NCBI GeneID: 7942
• NCBI Accession #: NP_009093.1
• NCBI GenBank Nucleotide #: NM_007162
• NCBI Official Full Name: Homo sapiens transcription factor EB (TFEB), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• Protein Family: Transcription factor
• UniProt Gene Name: TFEB
• UniProt Entry Name: TFEB_HUMAN

Uniprot Description

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: embryonic placenta development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; humoral immune response