Mouse anti-Human CBLB Monoclonal Antibody | anti-CBLB antibody

Mouse monoclonal antibody Anti-Human CBLB

Cat. Num. AAA120301

• Gene Names: CBLB; Cbl-b; RNF56; Nbla00127
• Reactivity: Human
• Applications: Western Blot
• Synonyms: CBLB; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human CBLB; Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence b; RNF56; Nbla00127; anti-CBLB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 246C5a
• Form/Format: Mouse monoclonal anti-human CBLB antibody in PBS (3.0 mM KCI, 1.5 mM KH₂PO₄, 140mM NaCI, 8.0 mM Na₂HPO₄ (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.0.5% sodium azide (NaN₃).
• Concentration: 0.1mg/ml (varies by lot)
• Sequence Length: 3923

• Applicable Applications for anti-CBLB antibody: Western Blot (WB)
• Application Notes: WB: 0.2-2 ug/ml
• Preparation: This antibody was purified using protein A column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃as a preservative. There is a potential hazard that NaN₃ reacts with copper or lead to produce an explosive compound.
• Immunogen: Recombinant
• Safety Warnings and precautions: Caution must be taken to avoid contact with skin or eyes. In such case, rinse thoroughly at once with water. Do not ingest, inhale or swallow. Seek medical attention immediately.; Wear appropiate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it it handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. ; The vial is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8°C for up to one year.; We recommend storing at -20°C for long term storage.; Avoid repeat freezing and thawing cycles.

Testing Data

(Western blot analysis of immunized recombinant protein, using anti-CBLB monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of CBLB by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. 246C5a-1] Predicted molecular weight: 109 kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 34192827
• NCBI GeneID: 868
• NCBI Official Full Name: Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence b, mRNA
• NCBI Official Synonym Full Names: Cbl proto-oncogene B, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBLB
• NCBI Official Synonym Symbols: Cbl-b; RNF56; Nbla00127
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL-B; Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Cbl proto-oncogene, E3 ubiquitin protein ligase B; RING finger protein 56; SH3-binding protein CBL-B; casitas B-lineage lymphoma proto-oncogene b; signal transduction protein CBL-B
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL-B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CBLB
• UniProt Entry Name: CBLB_HUMAN

Uniprot Description

Cbl-b: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Adaptor/scaffold; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Calcium-binding

Chromosomal Location of Human Ortholog: 3q13.11

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: signal transducer activity; protein binding; phosphotyrosine binding; zinc ion binding; ubiquitin-protein ligase activity; calcium ion binding; ligase activity

Biological Process: cell surface receptor linked signal transduction; T cell activation; positive regulation of protein catabolic process; negative regulation of T cell receptor signaling pathway; protein ubiquitination; negative regulation of alpha-beta T cell proliferation; immune response; NLS-bearing substrate import into nucleus; signal transduction; positive regulation of T cell anergy