Mouse anti-Human EXOSC9 Monoclonal Antibody | anti-EXOSC9 antibody

Mouse monoclonal antibody Anti-Human EXOSC9

Cat. Num. AAA120326

• Gene Names: EXOSC9; p5; p6; RRP45; PMSCL1; Rrp45p; PM/Scl-75
• Reactivity: Human
• Synonyms: EXOSC9; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human EXOSC9; Homo sapiens exosome component 9; p5; p6; RRP45;PMSCL1; Rrp45p; PM/Scl-75; anti-EXOSC9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2337C3a
• Form/Format: Mouse monoclonal anti-human EXOSC9 antibody in PBS (3.0mM KCI, 1.5 mM KH₂HPO₄ (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃)
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 1644

• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Safety Warning and Precautions: Caution must be taken to avoid contact with skin or eyes . In such a case, rinse thoughly at once with water. Do not ingest, inhale , or swallow. Seek medical attention inmediately. Wear appropiate protective clothing such as laboratory overalls, safety glass and gloves. It is strongly advised that this product should be handled by people who have bee well trained in laboratory tecniques and that it is handled with care pursuant to the principles of good laboratory practice . All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃) as a preservative. There is a pontenial hazard that NaN₃ reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed throughly with water.
• Immunogen: Recombinant
• Preparation and Storage: Store at 2-8°C. We recommend storing at -20°C for long term storage. Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-EXOSC9 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Related Product Information for anti-EXOSC9 antibody

Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity (By similarity). [UniProtKB Function]

NCBI and Uniprot Product Information

• NCBI GI #: 77812671
• NCBI GeneID: 5393
• NCBI Accession #: NP_001029366.1
• NCBI GenBank Nucleotide #: NM_001034194
• NCBI Official Full Name: Homo sapiens exosome component 9 (EXOSC9), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: exosome component 9
• NCBI Official Symbol: EXOSC9
• NCBI Official Synonym Symbols: p5; p6; RRP45; PMSCL1; Rrp45p; PM/Scl-75
• NCBI Protein Information: exosome complex component RRP45; P75 polymyositis-scleroderma overlap syndrome associated autoantigen; P75 polymyositis-scleroderma overlap syndrome-associated autoantigen; PMSCL autoantigen, 75kD; autoantigen PM/Scl 1; exosome complex exonuclease RRP45; polymyositis/scleroderma autoantigen 1, 75kDa
• UniProt Protein Name: Exosome complex component RRP45
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC9
• UniProt Entry Name: EXOS9_HUMAN

NCBI Description

This gene encodes a component of the human exosome, a exoribonuclease complex which processes and degrades RNA in the nucleus and cytoplasm. This component may play a role in mRNA degradation and the polymyositis/scleroderma autoantigen complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

EXOSC9: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs. Belongs to the RNase PH family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; RNA processing; Nucleolus

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: nucleoplasm; nuclear chromosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; exosome (RNase complex); nucleus; cytosol; nuclear exosome (RNase complex)

Molecular Function: 3'-5'-exoribonuclease activity; protein binding; exoribonuclease activity; RNA binding; AU-rich element binding

Biological Process: gene expression; immune response; positive regulation of cell growth; rRNA processing; mRNA catabolic process, deadenylation-dependent decay