Mouse anti-Human ATF3 Monoclonal Antibody | anti-ATF3 antibody

Mouse monoclonal antibody Anti-Human ATF3

Cat. Num. AAA120339

• Reactivity: Human
• Applications: Immunocytochemistry
• Synonyms: ATF3; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human ATF3; Homo sapiens activating transcription factor 3; anti-ATF3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 44C3a
• Form/Format: Mouse Monoclonal anti human ATF3 antibody in PBS (3.0 mM KCl, 1.5mM KH₂PO₄, 140 mM NaCl, 8.0 mM Na₂HPO₄ (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃
• Sequence Length: 124

• Applicable Applications for anti-ATF3 antibody: Immunocytochemistry (ICC)
• Application Notes: ICC: 2-100 ug/ml
• Preparation: This antibody was purified using protein A column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃)as a preservative. There is a potential hazard that NaN₃ reacts with copper or lead to produc an explosive compound. For safe disposal, the vial has to be washed thorughly with water.
• Safety warning and precautions: Caution must be taken to avoid contact with skin or eyes. In such case, rinse thoroughly at once with water. Do not ingest, inhale or swallow. Seek medical attention inmediately. Wear appropiate protective clothing such as laboratory overalls, safety glasess and gloves. It is strongly advised that this product should be handled by people who have been well traied in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8°C for up to one year.; We recommend storing at -20°C for long term storage.; Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-ATF3 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ATF3 mAb. )

Related Product Information for anti-ATF3 antibody

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 95102480
• NCBI GeneID: 467
• NCBI Accession #: NP_004015.4
• NCBI GenBank Nucleotide #: NM_004024
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor
• UniProt Gene Name: ATF3
• UniProt Entry Name: ATF3_HUMAN

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Uniprot Description

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription factor activity; transcription corepressor activity

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis