Western Blot (WB)
(Figure 1:The cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Figure 2:The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate)
Mouse anti-Human Casein Kinase 1 alpha Monoclonal Antibody | anti-CSNK1A1 antibody
Casein Kinase 1 alpha antibody
Gene Names
CSNK1A1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
Casein Kinase 1 alpha; Monoclonal Antibody; Casein Kinase 1 alpha antibody; CK1; anti-CSNK1A1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
AT2E2
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
337
Applicable Applications for anti-CSNK1A1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage , aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Figure 1:The cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Figure 2:The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate)
Western Blot (WB)
(Figure 1:The cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Figure 2:The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of Casein Kinase 1 alpha in HeLa cells line, stained with MBS200165 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stainied the cell nucleus (blue))
Immunofluorescence (IF)
(ICC/IF analysis of Casein Kinase 1 alpha in HeLa cells line, stained with MBS200165 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stainied the cell nucleus (blue))
Related Product Information for anti-CSNK1A1 antibody
Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex
Product Categories/Family for anti-CSNK1A1 antibody
References
Knippschild U, et al., (2005) Onkologie. 28 (10):508-14; Katoh M., (2007) Clin Cancer Res. 13 (14):4042-5
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
casein kinase I isoform alpha isoform 2
NCBI Official Synonym Full Names
casein kinase 1 alpha 1
NCBI Official Symbol
CSNK1A1
NCBI Official Synonym Symbols
CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
NCBI Protein Information
casein kinase I isoform alpha
UniProt Protein Name
Casein kinase I isoform alpha
Protein Family
UniProt Gene Name
CSNK1A1
UniProt Synonym Gene Names
CKI-alpha
Uniprot Description
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).
Research Articles on CSNK1A1
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Product Notes
The CSNK1A1 csnk1a1 (Catalog #AAA200165) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Casein Kinase 1 alpha antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Casein Kinase 1 alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CSNK1A1 csnk1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Casein Kinase 1 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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