Mouse anti-Human NAB1 Monoclonal Antibody | anti-NAB1 antibody

Mouse monoclonal antibody Anti-Human NAB1

Cat. Num. AAA120377

• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry, Western Blot
• Synonyms: NAB1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human NAB1; Homo sapiens NGFI-A binding protein 1 (EGR1 binding protein 1); anti-NAB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2607C2a
• Form/Format: Mouse monoclonal anti-human NAB1 antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN ).
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 4499

• Applicable Applications for anti-NAB1 antibody: Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Immunogen: Recombinant
• Disposal: This antibody solution contains sodium azide (NaN )as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Safety warnings and precautions: Caution must be taken to avoid contact with skin or eyes. ; In such a case, rinse thoroughly at once with water.; Do not ingest, inhale, or swallow. Seek medical attention immediately.; Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves.; It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice.; All chemicals are deemed potentially harmful.; The vial is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8 C for up to one year.; We recommend storing at –20 C for long-term storage.; Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-NAB1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of NAB1 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2607C2a-1]Predicted molecular weight: 54 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NAB1 mAb. [Lot No. 2607C2a-11])

Related Product Information for anti-NAB1 antibody

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2 (By similarity). [UniProtKB Function]

NCBI and Uniprot Product Information

• NCBI GI #: 56550107
• NCBI GeneID: 4664
• NCBI Accession #: NP_005957.2
• NCBI GenBank Nucleotide #: NM_005966
• Molecular Weight: 51,233 Da
• NCBI Official Full Name: Homo sapiens NGFI-A binding protein 1 (EGR1 binding protein 1) (NAB1), mRNA
• NCBI Official Synonym Full Names: NGFI-A binding protein 1 (EGR1 binding protein 1)
• NCBI Official Symbol: NAB1
• NCBI Protein Information: NGFI-A-binding protein 1; EGR-1-binding protein 1; EGR1 binding protein 1; transcriptional regulatory protein p54
• UniProt Protein Name: NGFI-A-binding protein 1
• Protein Family: NGFI-A-binding protein
• UniProt Gene Name: NAB1
• UniProt Entry Name: NAB1_HUMAN

Uniprot Description

NAB1: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Belongs to the NAB family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q32.3-q33

Cellular Component: nucleus

Molecular Function: transcription factor binding

Biological Process: Schwann cell differentiation; myelination; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; regulation of epidermis development; endochondral ossification