Mouse anti-Human NR4A1 Monoclonal Antibody | anti-NR4A1 antibody

Mouse monoclonal antibody Anti-Human NR4A1

Cat. Num. AAA120078

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry
• Synonyms: NR4A1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human NR4A1; Homo sapiens nuclear receptor subfamily 4; group A; member 1 (NR4A1); transcript variant 1; mRNA; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; anti-NR4A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: NR4AB9G12
• Form/Format: Mouse monoclonal anti-human NR4A1 antibody in PBS (3.0 mM KCI, 1.5 mM KH₂PO₄,140 mM NaCI, 8.0 mM KH₂PO₄(pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃)
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 2692

• Applicable Applications for anti-NR4A1 antibody: Dot Blot (DB), Immunocytochemistry (ICC)
• Application Notes: ICC : 2-100 ug/ml
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Immunogen: Recombinant
• Safety warnings and precautions: Caution must be taken to avoid contact with skin or eyes.In such a case, rinse thoughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advise that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃) as a preservative. There is a potential hazard that (NaN₃) reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Preparation and Storage: Store at 2-8°C for up to one year.; We recommend storing at -20°C for long-term storage. Avoid repeat freezing and thawing cycles

Western Blot (WB)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NR4A1 mAb. [Lot No.NR4AB9G12-2])

Related Product Information for anti-NR4A1 antibody

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this gene. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 320202953
• NCBI GeneID: 3164
• NCBI Accession #: NP_002126.2
• NCBI GenBank Nucleotide #: NM_002135
• NCBI Official Full Name: Homo sapiens nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1; ST-59; TR3 orphan receptor; early response protein NAK1; growth factor-inducible nuclear protein N10; hormone receptor; nerve growth factor IB nuclear receptor variant 1; nuclear hormone receptor NUR/77; orphan nuclear receptor HMR; orphan nuclear receptor TR3; steroid receptor TR3; testicular receptor 3
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Entry Name: NR4A1_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: Apoptosis; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; cell migration during sprouting angiogenesis; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression