Rabbit anti-Human TFEB Polyclonal Antibody | anti-TFEB antibody

TFEB, ID (TFEB, BHLHE35, Transcription factor EB, Class E basic helix-loop-helix protein 35) (PE)

Cat. Num. AAA6355073

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TFEB; Polyclonal Antibody; ID (TFEB; BHLHE35; Transcription factor EB; Class E basic helix-loop-helix protein 35) (PE); Class E basic helix-loop-helix protein 35; anti-TFEB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TFEB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TFEB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human TFEB.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TFEB Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TFEB antibody detected the TFEB protein (arrow).)

Related Product Information for anti-TFEB antibody

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.

Product Categories/Family for anti-TFEB antibody

Antibodies; Transcription Factors

References

Martignoni, G., et al. Mod. Pathol. 22(8):1016-1022(2009). Sardiello, M., et al. Science 325(5939):473-477(2009). Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008). Pecciarini, L., et al. Genes Chromosomes Cancer 46(5):419-426(2007). Argani, P., et al. Am. J. Surg. Pathol. 29(2):230-240(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 268607698
• NCBI GeneID: 7942
• NCBI Accession #: NP_009093.1
• NCBI GenBank Nucleotide #: NM_007162
• UniProt Accession #: P19484; Q709B3; Q7Z6P9; Q9BRJ5; Q9UJD8
• NCBI Official Full Name: Homo sapiens transcription factor EB (TFEB), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• Protein Family: Transcription factor
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN

Uniprot Description

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: embryonic placenta development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; humoral immune response; positive regulation of autophagy