Western Blot (WB)
(Western blot analysis of extracts from HT-29 and HeLa cells using MBS6010267.)
Rabbit DHX29 Monoclonal Antibody
DHX29 (ATP-dependent RNA Helicase DHX29, DEAH Box Protein 29, Nucleic Acid Helicase DDXx, DDX29)
Reactivity
Human, Monkey, Mouse
Applications
Western Blot, Immunoprecipitation
Purity
SupernatantSUpernatant
Synonyms
DHX29; Monoclonal Antibody; DHX29 (ATP-dependent RNA Helicase DHX29; DEAH Box Protein 29; Nucleic Acid Helicase DDXx; DDX29); Anti -DHX29 (ATP-dependent RNA Helicase DHX29; anti-DHX29 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
D19E10
Specificity
Recognizes endogenous levels of total human DHX29. Species Crossreactivity: Monkey and mouse.
Purity/Purification
Supernatant
SUpernatant
SUpernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-DHX29 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human DHX29.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from HT-29 and HeLa cells using MBS6010267.)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 and HeLa cells using MBS6010267.)
Related Product Information for anti-DHX29 antibody
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).
Product Categories/Family for anti-DHX29 antibody
Similar Products
Product Notes
The DHX29 (Catalog #AAA6010267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX29 (ATP-dependent RNA Helicase DHX29, DEAH Box Protein 29, Nucleic Acid Helicase DDXx, DDX29) reacts with Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHX29 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the DHX29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX29, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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