Rabbit CLK3 Polyclonal Antibody | anti-CLK3 antibody

CLK3 (cdc2-like Kinase 3)

Cat. Num. AAA617581

• Gene Names: CLK3; PHCLK3; FLJ22858; PHCLK3/152
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: CLK3; Polyclonal Antibody; CLK3 (cdc2-like Kinase 3); Anti -CLK3 (cdc2-like Kinase 3); anti-CLK3 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of full-length and truncated forms of CLK3 protein. Species Crossreactivity: Human, monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

• Applicable Applications for anti-CLK3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot.
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding His51 of CLK3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, HT29 and PC12 cell lines using MBS617581)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HT-29 cells using MBS617581 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

Related Product Information for anti-CLK3 antibody

The cdc2-like kinase (CLK) family contains at least four highly conserved isoforms CLK1, CLK2, CLK3 and CLK4 (1,2). CLKs are dual specific kinases that have been shown to autophosphorylate on serine, threonine and tyrosine residues and phosphorylate exogenous substrates on serine and threonine residues (2). CLK family members can co-exist as full-length catalytically active forms and an alternatively-spliced truncated inactive form (1). A family of highly phosphorylated proteins, so called serine- and arginine-rich (SR) proteins, have been shown to be phosphorylated by CLKs (3-5). SR proteins are splicing factors that regulate the assembly of the spliceosome, a macromolecular complex where RNA splicing occurs in the nucleus, and selection of splice sites. Thus, CLKs may play important roles in regulating RNA splicing.

CLK3 is abundantly expressed in the testis and, similar to other family members, has been implicated in regulating RNA splicing (6-8).

Product Categories/Family for anti-CLK3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18044742
• NCBI GeneID: 1198
• UniProt Accession #: P49761; Q53Y48; Q9BRS3; Q9BUJ7; D3DW59; Q59FC5
• Molecular Weight: 73,515 Da
• NCBI Official Full Name: CLK3 protein
• NCBI Official Synonym Full Names: CDC-like kinase 3
• NCBI Official Symbol: CLK3
• NCBI Official Synonym Symbols: PHCLK3; FLJ22858; PHCLK3/152
• NCBI Protein Information: dual specificity protein kinase CLK3; OTTHUMP00000180678; OTTHUMP00000233457
• UniProt Protein Name: Dual specificity protein kinase CLK3
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK3
• UniProt Entry Name: CLK3_HUMAN

NCBI Description

This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq]

Uniprot Description

CLK3: a CMGC kinase of the CLK family. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May play a role in regulating RNA splicing. Three alternatively spliced isoforms have been described. Isoform 2 is short and lacks the kinase domain. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: EC 2.7.12.1; Kinase, protein; Protein kinase, dual-specificity (non-receptor); Protein kinase, CMGC; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; membrane; acrosome; nuclear speck; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; regulation of RNA splicing; protein amino acid autophosphorylation; protein amino acid phosphorylation