Western Blot (WB)
(Western blot analysis of extracts of HeLa, A431 and H3255 cells using MBS613594 RecQ4 Antibody.)
Rabbit RecQ protein-like 4 Polyclonal Antibody | anti-RECQL4 antibody
RecQ protein-like 4 (ATP-dependent DNA helicase Q4, RecQ4, RECQ4, RTS)
Gene Names
RECQL4; RECQ4; RECQL4
Reactivity
Human, Monkey, Rat
Applications
Western Blot
Purity
Purified by peptide affinity chromatography.
Synonyms
RecQ protein-like 4; Polyclonal Antibody; RecQ protein-like 4 (ATP-dependent DNA helicase Q4; RecQ4; RECQ4; RTS); Anti -RecQ protein-like 4 (ATP-dependent DNA helicase Q4; anti-RECQL4 antibody
Host
Rabbit
Reactivity
Human, Monkey, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of total RecQ4 protein. Species Crossreactivity: Human, rat, monkey.
Purity/Purification
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Applicable Applications for anti-RECQL4 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy terminus of human RecQ4.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts of HeLa, A431 and H3255 cells using MBS613594 RecQ4 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa, A431 and H3255 cells using MBS613594 RecQ4 Antibody.)
Related Product Information for anti-RECQL4 antibody
RECQL4 is a member of the RecQ family of DNA helicases that plays an important role in global genomic stability. There are five members of this family in humans, and mutations in three of these, BLM, WRN and RECQL4, give rise to disorders that are characterized by premature aging and a predisposition to cancer (1). Despite the presence of a helicase domain, no helicase activity has been reported for RECQL4. Rather, RECQL4 has an ATPase function that is stimulated by ssDNA, and a ssDNA annealing activity that is inhibited by RPA (2). RECQL4 has been reported to interact with ubiquitin ligases UBR1 and UBR2 (3). The role of RECQL4 in tumor suppression and the maintenance of genomic integrity has been attributed to it's activities associated with the regulation of DNA replication, and DNA recombination and repair (4-6). Mutations in the RECQL4 gene have been identified in a subset of patients with Rothmund-Thomson syndrome (RTS) a disorder characterized by growth deficiency, skin and skeletal abnormalities, and cancer predisposition. Two more autosomal recessive disorders have been associated with RECQL4 gene mutations: RAPADILINO, and BallerGerold syndromes (4).
Product Categories/Family for anti-RECQL4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
133,077 Da
NCBI Official Full Name
RecQ protein-like 4
NCBI Official Synonym Full Names
RecQ protein-like 4
NCBI Official Symbol
RECQL4
NCBI Official Synonym Symbols
RECQ4; RECQL4
NCBI Protein Information
ATP-dependent DNA helicase Q4; DNA helicase, RecQ-like type 4; DNA helicase, RecQ-like, type 4
UniProt Protein Name
ATP-dependent DNA helicase Q4
Protein Family
UniProt Gene Name
RECQL4
UniProt Synonym Gene Names
RECQ4
UniProt Entry Name
RECQ4_HUMAN
NCBI Description
The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq]
Uniprot Description
RECQL4: DNA-dependent ATPase. May modulate chromosome segregation. Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity. Up-regulated in actively dividing cells. Ubiquitously expressed, with highest levels in thymus and testis. Belongs to the helicase family. RecQ subfamily.
Protein type: EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: membrane; cytoplasm; nucleus
Molecular Function: ATP-dependent 3'-5' DNA helicase activity; zinc ion binding; bubble DNA binding; ATP binding
Biological Process: multicellular organismal development; DNA strand renaturation; DNA repair; DNA replication; DNA duplex unwinding; DNA recombination
Disease: Rapadilino Syndrome; Rothmund-thomson Syndrome; Baller-gerold Syndrome
Research Articles on RECQL4
1. These results indicate that RECQ1 and RECQ4 are integral components of the human replication complex and play distinct roles in DNA replication initiation and replication fork progression in vivo.
2. RECQL4 specifically stimulates the apurinic endonuclease activity of APE1, the DNA strand displacement activity of DNA polymerase beta, and incision of a 1- or 10-nucleotide flap DNA substrate by Flap Endonuclease I.
3. Purified hRECQL4 protein (expressed in an insect expression system) is associated with both DNA helicase activity and single-stranded DNA-dependent ATPase activity.
4. MCM10 interacts directly with RECQ4 and regulates its DNA unwinding activity.
5. Assembly of the Cdc45-Mcm2-7-GINS complex requires the Ctf4/And-1, RecQL4, and Mcm10 proteins.
6. p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization.
7. All osteosarcomas overexpressed RECQL4 in comparison to controls
8. RECQ4 exhibits DNA helicase activity. Importantly, two distinct regions of the protein, the conserved helicase motifs and the Sld2-like N-terminal domain, each independently promote ATP-dependent DNA unwinding.
9. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
10. Study reports that RAPADILINO patients identified as carriers of the c.1390+2delT mutation (p.Ala420_Ala463del) are at increased risk to develop lymphoma or osteosarcoma (6 out of 15 patients).
2. RECQL4 specifically stimulates the apurinic endonuclease activity of APE1, the DNA strand displacement activity of DNA polymerase beta, and incision of a 1- or 10-nucleotide flap DNA substrate by Flap Endonuclease I.
3. Purified hRECQL4 protein (expressed in an insect expression system) is associated with both DNA helicase activity and single-stranded DNA-dependent ATPase activity.
4. MCM10 interacts directly with RECQ4 and regulates its DNA unwinding activity.
5. Assembly of the Cdc45-Mcm2-7-GINS complex requires the Ctf4/And-1, RecQL4, and Mcm10 proteins.
6. p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization.
7. All osteosarcomas overexpressed RECQL4 in comparison to controls
8. RECQ4 exhibits DNA helicase activity. Importantly, two distinct regions of the protein, the conserved helicase motifs and the Sld2-like N-terminal domain, each independently promote ATP-dependent DNA unwinding.
9. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
10. Study reports that RAPADILINO patients identified as carriers of the c.1390+2delT mutation (p.Ala420_Ala463del) are at increased risk to develop lymphoma or osteosarcoma (6 out of 15 patients).
Similar Products
Product Notes
The RECQL4 recql4 (Catalog #AAA613594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RecQ protein-like 4 (ATP-dependent DNA helicase Q4, RecQ4, RECQ4, RTS) reacts with Human, Monkey, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RecQ protein-like 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the RECQL4 recql4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RecQ protein-like 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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