Western Blot (WB)
(Western blot analysis of extracts from mouse and rat brain using MBS6011882.)
Rabbit BRSK2 Monoclonal Antibody
BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
SupernatantSupernatant
Synonyms
BRSK2; Monoclonal Antibody; NT (BR Serine/threonine Kinase 2; Serine/Threonine-protein Kinase 29; Serine/Threonine-protein Kinase SAD-A; C11orf7; PEN11B; STK29; HUSSY-12); Anti -BRSK2; anti-BRSK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
D29B6
Specificity
Recognizes endogenous levels of total human BRSK2. Species Crossreactivity: Mouse and rat.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-BRSK2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunogen
Synthetic peptide corresponding to residues near the amino terminus of human BRSK2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from mouse and rat brain using MBS6011882.)
Western Blot (WB)
(Western blot analysis of extracts from mouse and rat brain using MBS6011882.)
Related Product Information for anti-BRSK2 antibody
Brain-specific kinase 1 (BRSK1; SAD-B) and Brain-specific kinase 2 (BRSK2; SAD-A) are serine/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of -tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -/- and BRSK2 -/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 in vitro diminishes axonal growth (5).
Product Categories/Family for anti-BRSK2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The BRSK2 (Catalog #AAA6011882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRSK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the BRSK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRSK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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