Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS612781.)
Rabbit Eukaryotic Translation Initiation Factor 4A Polyclonal Antibody | anti-Eif-4a antibody
Eukaryotic Translation Initiation Factor 4A (eIF4A)
Reactivity
Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A and immunoaffinity chromatography.
Synonyms
Eukaryotic Translation Initiation Factor 4A; Polyclonal Antibody; Eukaryotic Translation Initiation Factor 4A (eIF4A); Anti -Eukaryotic Translation Initiation Factor 4A (eIF4A); anti-Eif-4a antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins.
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.
Applicable Applications for anti-Eif-4a antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Synthetic peptide (KLH-coupled) derived from residues surrounding Phe52 of human eIF4A
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS612781.)
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS612781.)
Related Product Information for anti-Eif-4a antibody
A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).
Product Categories/Family for anti-Eif-4a antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48kD
NCBI Official Full Name
eukaryotic translation initiation factor 4A
NCBI Official Symbol
Eif-4a
NCBI Protein Information
eukaryotic translation initiation factor 4A
Protein Family
Similar Products
Product Notes
The Eif-4a (Catalog #AAA612781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eukaryotic Translation Initiation Factor 4A (eIF4A) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Eukaryotic Translation Initiation Factor 4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the Eif-4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eukaryotic Translation Initiation Factor 4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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