Rabbit GLAST Monoclonal Antibody | anti-Glast antibody

GLAST (Excitatory Amino Acid Transporter 1, Sodium-dependent Glutamate/Aspartate Transporter 1, GLAST-1, Solute Carrier Family 1 Member 3, SLC1A3, EAAT1, GLAST1)

Cat. Num. AAA6000798

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Supernatant; Supernatant
• Synonyms: GLAST; Monoclonal Antibody; GLAST (Excitatory Amino Acid Transporter 1; Sodium-dependent Glutamate/Aspartate Transporter 1; GLAST-1; Solute Carrier Family 1 Member 3; SLC1A3; EAAT1; GLAST1); Anti -GLAST (Excitatory Amino Acid Transporter 1; anti-Glast antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: D20D5
• Specificity: Recognizes endogenous levels of total human EAAT1. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Glast antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Glu230 of human EAAT1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from mouse and rat brain using EAAT1 rabbit mAb.)

Related Product Information for anti-Glast antibody

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3). EAAT2 accounts for up to 90% of the total glutamate transport in brain while EAAT1 contributes the remaining 5-10% (4). The contribution of EAAT1 in neurotransmission is unclear since EAAT2 is much more abundant. However, EAAT1 expression is upregulated by increasing concentrations of glutamate in the media of cultured primary astrocytes, potentially giving this glutamate transporter additional importance (5). EAAT1 has neuroprotective potential following ischemia since reactive astrocytes and activated microglia express EAAT1 but not EAAT2 (6).

Product Categories/Family for anti-Glast antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 350539797
• NCBI GeneID: 100689464
• NCBI Accession #: NP_001233651.1
• NCBI GenBank Nucleotide #: NM_001246722.1
• NCBI Official Full Name: glutamate and aspartate transporter
• NCBI Official Symbol: Glast
• NCBI Protein Information: glutamate and aspartate transporter