Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS619994.)
Rabbit DHX29 Polyclonal Antibody | anti-DHX29 antibody
DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)
Gene Names
DHX29; DDX29; FLJ21492
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
DHX29; Polyclonal Antibody; DHX29 (DEAH (Asp-Glu-Ala-His) Box 29; DDX29; FLJ21492; Nucleic acid helicase DDX); Anti -DHX29 (DEAH (Asp-Glu-Ala-His) Box 29; anti-DHX29 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human DHX29 (G63). Species crossreactivity: mouse, rat, monkey.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Applicable Applications for anti-DHX29 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunogen
Synthetic peptide derived from a sequence around Gly63 of human DHX29 protein (KLH-coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS619994.)
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS619994.)
Related Product Information for anti-DHX29 antibody
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).
Product Categories/Family for anti-DHX29 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
155,236 Da
NCBI Official Full Name
DEAH (Asp-Glu-Ala-His) box polypeptide 29
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 29
NCBI Official Symbol
DHX29
NCBI Official Synonym Symbols
DDX29; FLJ21492
NCBI Protein Information
ATP-dependent RNA helicase DHX29; OTTHUMP00000221887; DEAH box protein 29; nucleic acid helicase DDXx; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29
UniProt Protein Name
ATP-dependent RNA helicase DHX29
Protein Family
UniProt Gene Name
DHX29
UniProt Synonym Gene Names
DDX29
UniProt Entry Name
DHX29_HUMAN
Uniprot Description
DDX29: ATP-binding RNA helicase involved in translation initiation. Required for efficient initiation on mammalian mRNAs with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. Belongs to the DEAD box helicase family. DEAH subfamily.
Protein type: Translation; Helicase; EC 3.6.4.13
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: mitochondrion; nucleus
Molecular Function: translation initiation factor activity; ATP-dependent RNA helicase activity; ribosomal small subunit binding; ATP binding
Biological Process: RNA processing; translational initiation
Research Articles on DHX29
Similar Products
Product Notes
The DHX29 dhx29 (Catalog #AAA619994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DHX29 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Researchers should empirically determine the suitability of the DHX29 dhx29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
