Rabbit Plk1 Polyclonal Antibody | anti-PLK1 antibody

Plk1, phosphorylated (Thr210) (Polo-like Kinase 1, PLK-1, PLK, Serine/threonine-protein Kinase PLK1, Serine/threonine-protein Kinase 13, STPK13)

Cat. Num. AAA645464

• Reactivity: Human, Monkey
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Plk1; Polyclonal Antibody; phosphorylated (Thr210) (Polo-like Kinase 1; PLK-1; PLK; Serine/threonine-protein Kinase PLK1; Serine/threonine-protein Kinase 13; STPK13); Anti -Plk1; anti-PLK1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels PLK1, phosphorylated at (thr210). Species Crossreactivity: monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-PLK1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr210 of human PLK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from HT-29 cells, asynchronous or synchronized in mitosis using Phospho-PLK1 (Thr210) Antibody #MBS645464. The antibody was pre-incubated with a PLK1 phospho-Thr210 peptide or nonphospho-peptide as indicated. The same lysates were examined using total PLK1 Rabbit mAb. Mitotic synchronization was performed by thymidine block followed by release into nocodazole and mitotic shake-off.)

Related Product Information for anti-PLK1 antibody

At least 4 distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3 and PLK4/SAK (1). PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133 causing translocation of these proteins from the cytoplasm to the nucleus (2-5). PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to inactivate Myt1, one of the kinases known to phosphorylate cdc2 at Thr14/Tyr15 (6). Polo-like kinases also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres (7). Mitotic exit requires activation of the anaphase promoting complex (APC) (8), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A and cdc20 (9). PLK1 phosphorylation of the APC subunits Apc1, cdc16 and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated (10,11).

Product Categories/Family for anti-PLK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 548520203
• NCBI GeneID: 102171678
• NCBI Accession #: XP_005697666.1
• NCBI GenBank Nucleotide #: XM_005697609.1
• NCBI Official Full Name: serine/threonine-protein kinase PLK1 isoform X2
• NCBI Official Symbol: PLK1
• NCBI Protein Information: polo-like kinase 1
• Protein Family: Serine/threonine-protein kinase