Mouse anti-Human DHX29 Monoclonal Antibody | anti-DHX29 antibody

DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)

Cat. Num. AAA600888

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DHX29; Monoclonal Antibody; DHX29 (DEAH (Asp-Glu-Ala-His) Box 29; DDX29; FLJ21492; Nucleic acid helicase DDX); Anti -DHX29 (DEAH (Asp-Glu-Ala-His) Box 29; anti-DHX29 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C116
• Specificity: Recognizes human DHX29.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-DHX29 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.; Dilution: Flow Cytometry: 0.5-2ug/ml; Western Blot: 0.5-2ug/ml; Immunocytochemistry: 2-50ug/ml
• Immunogen: Partial sequence of recombinant full-length protein to human DHX29
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Western Blot (WB)

(Detection of human DHX29 by Western Blot. Samples: Whole cell lysate (25ug) from HeLa cells. Predicted molecular weight: 155kD)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DHX29 mAb.)

Testing Data

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DHX29 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)

Immunostaining (IS)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DHX29 mAb.)

Related Product Information for anti-DHX29 antibody

DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

Product Categories/Family for anti-DHX29 antibody

Antibodies; Abs to DEAH Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 329663671
• NCBI GeneID: 512470
• NCBI Accession #: NP_001193063.1
• NCBI GenBank Nucleotide #: NM_001206134.1
• UniProt Accession #: Q2YDN9
• Molecular Weight: 39,717 Da
• NCBI Official Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29
• NCBI Official Symbol: DHX29
• NCBI Protein Information: DEAH (Asp-Glu-Ala-His) box polypeptide 29; DEAH (Asp-Glu-Ala-His) box polypeptide 29
• UniProt Protein Name: DHX29 protein
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX29
• UniProt Entry Name: Q2YDN9_BOVIN