Rabbit anti-Human NKCC1 Polyclonal Antibody

NKCC1 (Sodium, Potassium, Chloride Cotransporter 1, BSC2, CCC1)

Cat. Num. AAA622039

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Purified by Protein A affinity chromatography.
• Synonyms: NKCC1; Polyclonal Antibody; NKCC1 (Sodium; Potassium; Chloride Cotransporter 1; BSC2; CCC1); Anti -NKCC1 (Sodium; anti-NKCC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NKCC1.
• Purity/Purification: Purified by Protein A affinity chromatography.
• Form/Format: Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-NKCC1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%; Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50.
• Immunogen: Synthetic peptide corresponding to residues surrounding Pro128 of human NKCC1 (blue carrier-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from DLD-1 and HT-29 cells using MBS622039.)

Related Product Information for anti-NKCC1 antibody

The electroneutral cation-chloride-coupled co-transporter (SLC12) gene family is comprised of bumetanide-sensitive Na+/K+/Cl- (NKCC), thiazide-sensitve Na+/Cl- and K+/Cl- (KCC) co-transporters. SLC12A1/NKCC2 and SLC12A2/NKCC1 regulate cell volume and maintain cellular homeostasis in response to osmotic and oxidative stress (1). The broadly expressed NKCC1 is thought to play roles in fluid secretion (i.e. salivary gland function), salt balance (i.e. maintenance of renin and aldosterone levels) and neuronal development and signaling (2-7). During neuronal development, NKCC1 and KCC2 maintain a fine balance between chloride influx (NKCC1) and efflux (KCC2), which regulates -aminobutyric acid (GABA)-mediated neurotransmission (3). Increased NKCC1 expression in immature neurons maintains high intracellular chloride levels that result in inhibitory GABAergic signaling; KCC2 maintains low intracellular chloride levels and excitatory GABAergic responses in mature neurons (4,5,8). Deletion of NKCC1 impairs NGF-mediated neurite outgrowth in PC-12D cells while inhibition of NKCC1 with bumetanide inhibits re-growth of axotomized dorsal root ganglion cells (6,7). Defective chloride homeostasis in neurons is linked to seizure disorders that are ameliorated by butemanide treatment, indicating that abnormal NKCC1 and NKCC2 expression or signaling may play a role in neonatal and adult seizures (9-12).

NKCC1 is found as a homodimer or within heterooligomers with other SLC12 family members. This transport protein associates with a number of oxidative- and osmotic-responsive kinases that bind, phosphorylate and activate NKCC1 co-transporter activity (13-16). In response to decreased intracellular chloride concentrations, Ste20-related proline-alanine-rich kinase (SPAK) phosphorylates NKCC1 to increase co-transporter activity and promote chloride influx (16-19). Oxidative stress response kinase 1 (OSR1) also phosphorylates and activates NKCC1 in response to oxidative stress (14).

Product Categories/Family for anti-NKCC1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 336088781
• NCBI Official Full Name: NKCC1