Rabbit KDM1A Polyclonal Antibody | anti-KDM1A antibody

KDM1A Antibody

Cat. Num. AAA9410879

• Gene Names: KDM1A; AOF2; KDM1; LSD1; BHC110
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: KDM1A; Polyclonal Antibody; KDM1A Antibody; KDM1; AOF2; BHC110; KIAA0601; LSD1; anti-KDM1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total KDM1A protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 876

• Applicable Applications for anti-KDM1A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (CHIP)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200; Immunoprecipitation: 1:20 - 1:100; Chromatin immunoprecipitation: 1:20 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human KDM1A.
• Target Name: KDM1A
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lysates using KDM1A antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer using KDM1A antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney using KDM1A antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain using KDM1A antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney using KDM1A antibody at dilution of 1:100 (400x lens)..)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using KDM1A antibody. Blue: DAPI for nuclear staining)

Related Product Information for anti-KDM1A antibody

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).

Product Categories/Family for anti-KDM1A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 58761544
• NCBI GeneID: 23028
• NCBI Accession #: NP_001009999.1
• NCBI GenBank Nucleotide #: NM_001009999.2
• UniProt Accession #: O60341; Q5TH94; Q5TH95; Q86VT7; Q8IXK4; Q8NDP6; Q8TAZ3; Q96AW4; A8MWP9
• Molecular Weight: 95,155 Da
• NCBI Official Full Name: lysine-specific histone demethylase 1A isoform a
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 1A
• NCBI Official Symbol: KDM1A
• NCBI Official Synonym Symbols: AOF2; KDM1; LSD1; BHC110
• NCBI Protein Information: lysine-specific histone demethylase 1A
• UniProt Protein Name: Lysine-specific histone demethylase 1A
• Protein Family: Lysine-specific histone demethylase
• UniProt Gene Name: KDM1A
• UniProt Synonym Gene Names: AOF2; KDM1; KIAA0601; LSD1
• UniProt Entry Name: KDM1A_HUMAN

NCBI Description

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

LSD1: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Ubiquitously expressed. Belongs to the flavin monoamine oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription factor; Demethylase; EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; nucleus

Molecular Function: FAD binding; p53 binding; oxidoreductase activity; transcription factor binding; histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-K4 specific); protein binding; MRF binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; histone demethylase activity; androgen receptor binding; chromatin binding; transcription factor activity; demethylase activity

Biological Process: negative regulation of histone H3-K9 methylation; establishment and/or maintenance of chromatin architecture; granulocyte differentiation; transcription, DNA-dependent; in utero embryonic development; negative regulation of transcription factor activity; positive regulation of erythrocyte differentiation; muscle cell development; negative regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of DNA binding; protein amino acid demethylation; regulation of transcription from RNA polymerase II promoter; cell proliferation; negative regulation of DNA damage response, signal transduction by p53 class mediator; histone H3-K9 demethylation; pituitary gland development; positive regulation of hormone biosynthetic process; positive regulation of megakaryocyte differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; negative regulation of protein binding; negative regulation of transcription, DNA-dependent