Testing Data
(Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1 ug/ml, 24 hr; +), using Phospho-Histone H3 (Thr32) Rabbit pAb (upper) E12-2321:2000 and Histone H3 (1G1) Mouse mAb (lower) E12-055 1:2000)
Histone H3 Polyclonal Antibody | anti-H3 antibody
Histone H3 (Phospho Thr32) Rabbit Polyclonal Antibody
Testing Data
(Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1 ug/ml, 24 hr; +), using Phospho-Histone H3 (Thr32) Rabbit pAb (upper) E12-2321:2000 and Histone H3 (1G1) Mouse mAb (lower) E12-055 1:2000)
Testing Data
(Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1 ug/ml, 24 hr; +), using Phospho-Histone H3 (Thr32) Rabbit pAb (upper) E12-2321:2000 and Histone H3 (1G1) Mouse mAb (lower) E12-055 1:2000)
NCBI and Uniprot Product Information
Uniprot Description
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.CautionTo ensure consistency between histone entries, we follow the 'Brno' nomenclature for histone modifications, with positions referring to those used in the literature for the 'closest' model organism. Due to slight variations in histone sequences between organisms and to the presence of initiator methionine in UniProtKB/Swiss-Prot sequences, the actual positions of modified amino acids in the sequence generally differ. In this entry the following conventions are used: H3T3ph = phosphorylated Thr-4; H3K4me1/2/3 = mono-, di- and trimethylated Lys-5; H3K9ac = acetylated Lys-10; H3K9me1/2/3 = mono-, di- and trimethylated Lys-10; H3S10ph = phosphorylated Ser-11; H3T11ph = phosphorylated Thr-12; H3K14ac = acetylated Lys-15; H3K18ac = acetylated Lys-19; H3K18me = methylated Lys-19; H3K23ac = acetylated Lys-24; H3K23me = methylated Lys-24; H3K27me = methylated Lys-28; H3S28ph = phosphorylated Ser-29; H3K36me = methylated Lys-37.
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Product Notes
The Histone H3 h3 (Catalog #AAA8503145) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Histone H3 (Phospho Thr32) Rabbit Polyclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000-2,000. Researchers should empirically determine the suitability of the Histone H3 h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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