KAT2B/PCAF recombinant protein

Recombinant KAT2B/PCAF protein

Cat. Num. AAA389199

• Gene Names: KAT2B; P; CAF; PCAF; P/CAF
• Synonyms: KAT2B/PCAF; Recombinant KAT2B/PCAF protein; K (lysine) acetyltransferase 2B (KAT2B); P300/CBP-associated factor; P/CAF; KAT2B/PCAF recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant PCAF protein is suppiled in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. Assay Conditions: 0.5ug Histone H4 was incubated with 0ug (-), 0.5ug (+) PCAF in a 20ul reaction system containing 50mM Tris-HCl pH 8.0, 0.1mM EDTA, 50 ng/ul BSA, 1mM TCEP and 20uM Acetyl-CoA for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H4ac (pan-acetyl) antibody was used to recognize acetylated histone H4.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PCAF protein gel 7.5% SDS-PAGE Coomassie staining MW: 94.3 kDa Purity: >80%)

Western Blot (WB)

(Western blot for recombinant PCAF activity 0.5 ug Histone H4 was incubated with 0 ug (-), 0.5 ug (+) PCAF in a 20 ul reaction system for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H4ac (pan-acetyl) antibody was used to recognize acetylated histone H4.)

Related Product Information for KAT2B/PCAF recombinant protein

Short Description: Recombinant human PCAF was expressed in a baculovirus expression system as the full-length protein (accession number NP_003875.3) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 94.3 kDa. It is suitable for use in in binding assays, inhibitor screening, and selectivity profiling.

Background: p300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT's transactivating activity and may help induce chromatin remodeling of proviral genes.

Product Categories/Family for KAT2B/PCAF recombinant protein

Recombinant Proteins; Bromodomain

NCBI and Uniprot Product Information

• NCBI GI #: 40805843
• NCBI GeneID: 8850
• NCBI Accession #: NP_003875.3
• NCBI GenBank Nucleotide #: NM_003884.4
• UniProt Accession #: Q92831; Q6NSK1
• Molecular Weight: 93,013 Da
• NCBI Official Full Name: histone acetyltransferase KAT2B
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 2B
• NCBI Official Symbol: KAT2B
• NCBI Official Synonym Symbols: P; CAF; PCAF; P/CAF
• NCBI Protein Information: histone acetyltransferase KAT2B; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF
• UniProt Protein Name: Histone acetyltransferase KAT2B
• UniProt Gene Name: KAT2B
• UniProt Synonym Gene Names: PCAF; Histone acetylase PCAF; P/CAF
• UniProt Entry Name: KAT2B_HUMAN

NCBI Description

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

PCAF: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ubiquitously expressed but most abundant in heart and skeletal muscle. Belongs to the GCN5 family.

Protein type: Acetyltransferase; Nuclear receptor co-regulator; EC 2.3.1.48

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: kinetochore; nucleoplasm; I band; PCAF complex; actomyosin; nucleus; A band

Molecular Function: lysine N-acetyltransferase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; acetyltransferase activity; protein complex binding; transcription cofactor activity; transcription factor binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; establishment and/or maintenance of chromatin architecture; internal peptidyl-lysine acetylation; viral reproduction; negative regulation of cyclin-dependent protein kinase activity; rhythmic process; transcription from RNA polymerase I promoter; chromatin remodeling; negative regulation of cell proliferation; protein amino acid acetylation; cellular response to insulin stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; cell cycle arrest; peptidyl-lysine acetylation; N-terminal peptidyl-lysine acetylation