Typical Testing Data/Standard Curve (for reference only)
Human CD40L ELISA Kit | CD40L elisa kit
CD40L ELISA
Reactivity
Human
Applications
ELISA
Synonyms
CD40L; CD40L ELISA; CD40L elisa kit
Reactivity
Human
Specificity
This ELISA is specific for the measurement of natural and recombinant human CD40L [CD254].
Applicable Applications for CD40L elisa kit
ELISA (EIA)
Samples
Cell culture supernatants and serum
Detection Range
0.03125 ng/ml - 2 ng/ml
Sensitivity
20 pg/ml
Intra-assay Precision
Four samples of known concentrations of human CD40L were assayed in replicates 6 times to test precision within an assay.
Inter-assay Precision
Four samples of known concentrations of human CD40L were assayed in 5 separate assays to test precision between assays.
Preparation and Storage
Store at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD40L elisa kit
Intended Uses: The CD40L (human) ELISA Kit is to be used for the quantitative determination of human CD40L in cell culture supernatants and serum. This ELISA Kit is for research use only.
Background/Introduction: CD40 (also called Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5), a transmembrane type I glycoprotein, belongs to the tumor necrosis factor (TNF) gene superfamily and behaves as a co-stimulatory molecule found on B cells, monocytes, antigen presenting cells, endothelial, smooth muscle cells, fibroblasts and platelets (1, 2). Its ligand, CD40L (also called CD154), a type II transmembrane protein, is a 261 amino acid (aa) glycoprotein that forms homotrimers (3). CD40L is found on activated T cells, B cells, platelets, endothelial, epithelial and smooth muscle cells (3, 4). Proteolytic cleavage can produce a soluble form of CD40L (sCD40L) (4). Platelets are the main source of sCD40L (5). CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production and isotype switching, formation of B memory cells and germinal centers. It also prevents apoptosis of germinal center B cells (1-4). Defective expression of CD40L in humans leads to an inability to produce isotypes other than IgM (hyper IgM syndrome), and to an absence of germinal centers (2). In dendritic cells, CD40 ligation induces more effective antigen, enhances T-cell stimulatory capacity and induces production of several inflammatory cytokines and chemokines (6). CD40-CD40L interactions are crucially involved in development of autoimmune disease and have also impact on growth regulation of certain carcinomas (7). Elevated levels of sCD40L have been observed in sera from patients with systemic lupus erythematosus (SLE) (8), chronic lymphocytic leukemia (CLL) and unstable angina (1).
Background/Introduction: CD40 (also called Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5), a transmembrane type I glycoprotein, belongs to the tumor necrosis factor (TNF) gene superfamily and behaves as a co-stimulatory molecule found on B cells, monocytes, antigen presenting cells, endothelial, smooth muscle cells, fibroblasts and platelets (1, 2). Its ligand, CD40L (also called CD154), a type II transmembrane protein, is a 261 amino acid (aa) glycoprotein that forms homotrimers (3). CD40L is found on activated T cells, B cells, platelets, endothelial, epithelial and smooth muscle cells (3, 4). Proteolytic cleavage can produce a soluble form of CD40L (sCD40L) (4). Platelets are the main source of sCD40L (5). CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production and isotype switching, formation of B memory cells and germinal centers. It also prevents apoptosis of germinal center B cells (1-4). Defective expression of CD40L in humans leads to an inability to produce isotypes other than IgM (hyper IgM syndrome), and to an absence of germinal centers (2). In dendritic cells, CD40 ligation induces more effective antigen, enhances T-cell stimulatory capacity and induces production of several inflammatory cytokines and chemokines (6). CD40-CD40L interactions are crucially involved in development of autoimmune disease and have also impact on growth regulation of certain carcinomas (7). Elevated levels of sCD40L have been observed in sera from patients with systemic lupus erythematosus (SLE) (8), chronic lymphocytic leukemia (CLL) and unstable angina (1).
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Product Notes
The Human CD40L (Catalog #AAA490397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA490397 ELISA Kit recognizes Human CD40L. AAA Biotech's CD40L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the CD40L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40L, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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