Typical Testing Data/Standard Curve (for reference only)
Human PD-L1 ELISA Kit | PD-L1 elisa kit
PD-L1 (Human) ELISA
Reactivity
Human
Synonyms
PD-L1; PD-L1 (Human) ELISA; PD-L1 elisa kit
Reactivity
Human
Specificity
This ELISA is specific for the measurement of natural and recombinant human PD-L1 [CD274].
Samples
Cell culture supernatants, serum and plasma
Assay Type
Quantitative Sandwich
Detection Range
2.343 pg/ml - 150 pg/ml
Sensitivity
0.8 pg/ml
Intra-assay Precision
Four samples of known concentrations of human PD-L1 were assayed in replicates 6 times to test precision within an assay.
Inter-assay Precision
Four samples of known concentrations of human PD-L1 were assayed in 5 separate assays to test precision between assays.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PD-L1 elisa kit
Intended Uses: The PD-L1 (human) ELISA Kit is to be used for the quantitative determination of human PD-L1 in cell culture supernatants, serum and plasma. This ELISA Kit is for research use only.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human PD-L1 in cell culture supernatants, serum and plasma (EDTA, heparin or citrate). A monoclonal antibody specific for PD-L1 has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, PD-L1 is recognized by the addition of a biotinylated monoclonal antibody specific for PD-L1 (DET). After removal of excess biotinylated antibody, streptavidin-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of PD-L1 in the samples.
Background/Introduction: PD-L1, (also known as CD274 or B7-H1), is an immuno-coinhibitory member of the B7 family and functions as an immune checkpoint via its interaction with its receptors PD-1 (CD279) and CD80 (also known as B7.1) (1). PD-L1 is expressed by hematopoietic cells such as T, B and dendritic cells and by parenchymal cells in response to cytokine (i.e., IFN-gamma) induction. Ligation of PD-1 by PD-L1 (B7-H1; CD274) or PD-L2 (B7-DC; CD273) inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-gamma and also inhibits BCR mediated signaling (2). PD-L1 binding to CD80 inhibits T cells proliferation. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (3). A novel approach to fight cancer is to target immune checkpoint proteins with antibody inhibitors, such as anti-PD-1 or anti-PD-L1 that function as a tumor suppressing factor via the modulation of immune cell-tumor cell interactions (4). The soluble form of PD-L1 (sPD-L1) is expressed upon immune cells activation and can be secreted in the serum/plasma. Soluble checkpoints, such as sPD-1 or sPD-L1, are involved in positive or negative immune regulation and changes in their serum/plasma levels affect the development, prognosis and treatment of cancer (5). Increase of sPD-L1 or sPD-1 levels have been detected in samples with different types of cancer (6). This PD-L1 (human) ELISA Kit is developed to detect specifically human soluble PD-L1 in different biological fluids.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human PD-L1 in cell culture supernatants, serum and plasma (EDTA, heparin or citrate). A monoclonal antibody specific for PD-L1 has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, PD-L1 is recognized by the addition of a biotinylated monoclonal antibody specific for PD-L1 (DET). After removal of excess biotinylated antibody, streptavidin-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of PD-L1 in the samples.
Background/Introduction: PD-L1, (also known as CD274 or B7-H1), is an immuno-coinhibitory member of the B7 family and functions as an immune checkpoint via its interaction with its receptors PD-1 (CD279) and CD80 (also known as B7.1) (1). PD-L1 is expressed by hematopoietic cells such as T, B and dendritic cells and by parenchymal cells in response to cytokine (i.e., IFN-gamma) induction. Ligation of PD-1 by PD-L1 (B7-H1; CD274) or PD-L2 (B7-DC; CD273) inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-gamma and also inhibits BCR mediated signaling (2). PD-L1 binding to CD80 inhibits T cells proliferation. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (3). A novel approach to fight cancer is to target immune checkpoint proteins with antibody inhibitors, such as anti-PD-1 or anti-PD-L1 that function as a tumor suppressing factor via the modulation of immune cell-tumor cell interactions (4). The soluble form of PD-L1 (sPD-L1) is expressed upon immune cells activation and can be secreted in the serum/plasma. Soluble checkpoints, such as sPD-1 or sPD-L1, are involved in positive or negative immune regulation and changes in their serum/plasma levels affect the development, prognosis and treatment of cancer (5). Increase of sPD-L1 or sPD-1 levels have been detected in samples with different types of cancer (6). This PD-L1 (human) ELISA Kit is developed to detect specifically human soluble PD-L1 in different biological fluids.
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Product Notes
The Human PD-L1 (Catalog #AAA495597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495597 ELISA Kit recognizes Human PD-L1. It is sometimes possible for the material contained within the vial of "PD-L1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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