Typical Testing Data/Standard Curve (for reference only)
CD30L elisa kit
CD30L (Mouse) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse Caspase-1 (Caspase-1) in cell culture supernatants, serum and plasma. A monoclonal antibody specific for Caspase-1 has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Caspase-1 is recognized by the addition of a biotinylated monoclonal antibody specific for Caspase-1 (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of Caspase-1 in the samples!!Background/Introduction: Caspase-1 is the best-described inflammatory caspase (1). It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is synthesized as inactive pro-Caspase-1, wich is activated and processed by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes (2). NLRC4 responds to cytosolic flagellin (3), murine NLRP1b responds to anthrax lethal toxin (4), AIM2 responds to cytosolic DNA(5) and NLRP3/NALP3 responds to a variety of agonists including crystals (6). Pro-Caspase-1, once activated by an inflammasome complex is processed into p20 and p10 subunits that assembles to form a p20-p20 /p10-p10 active complex that is secreted by a nonconventional (non-ER-dependent) mechanism (7).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
TNFSF8: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Belongs to the tumor necrosis factor family.
Protein type: Cytokine; Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 9q33
Cellular Component: extracellular space; integral to plasma membrane
Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding
Biological Process: apoptosis; CD8-positive, alpha-beta T cell differentiation; cell proliferation; cell-cell signaling; defense response to Gram-positive bacterium; immune response; positive regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on CD30L
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Product Notes
The CD30L tnfsf8 (Catalog #AAA495206) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD30L, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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