Typical Testing Data/Standard Curve (for reference only)
IL-10 elisa kit
IL-10 ELISA Kit
Synonyms
IL-10; IL-10 ELISA Kit; Interleukin-10; IL-10 elisa kit
Specificity
This ELISA is specific for the measurement of natural and recombinant mouse IL-1alpha?(proform and p17 fragment). It does not cross-react with human IL-1alphaor mouse IL-beta.
Sequence Length
168
Samples
Cell Culture Supernatants, Serum And Plasma
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
9 pg/ml
Intra-assay Precision
Four samples of known concentrations of mouse IL-1alpha were assayed in replicates 7 times to test precision within an assay
Inter-assay Precision
Four samples of known concentrations of mouse IL-1alpha were assayed in 3 separate assays to test precision between assays.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-10 elisa kit
Intended Uses: The IL-1 alpha mouse ELISA Kit is to be used for the measurement of mouse IL-1alpha in cell culture supernatants, serum and plasma. This ELISA kit is for research use only.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse IL-1alpha (IL-1alpha) in cell culture supernatants, serum and plasma. A monoclonal antibody specific for IL-1alphahas been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, IL-1alphais recognized by the addition of a biotinylated monoclonal antibody specific for IL-1alpha?(DET). After removal of excess biotinylated antibody, streptavidin-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of IL-1alpha?in the samples!!Background/Introduction: The IL-1 family consists of two pro-inflammatory cytokines IL-1alpha, IL-1beta and the anti-inflammatory IL-1 receptor antagonist (IL-1Ra or IL-1RN). The two IL-1 isoforms (alpha and beta) are encoded by distinct genes but are structurally related and bind to the same receptors that exists in two major forms, Type I (IL-1R1) and Type II (IL-1R2) (1). The major biological roles of IL-1 are described as endogenous pyrogen, leukocyte endogenous mediator, lymphocyte, beta-cell, osteoclast, epidermal cell derived thymocyte activating factor, hematopoietin-1 and mononuclear cell factor (1). IL-1alpha plays a central role in cell growth, tissue repair and chronic inflammatory diseases (1). IL-1alpha is a unique member in the cytokine family which is synthesized as a 33 kDa precursor protein, ProIL-1alpha (1). The mature form p17 results by removal of N-terminal amino acids from ProIL-1alpha. Recently, it has been shown that the mature form p17 of IL-1alpha is the active cytokine. The ProIL-1alpha is poorly active due to its binding to the inhibitory intracellular IL-1R2 protein. Upon inflammasome activation, caspase-1 specifically cleaves IL-1R2, causing release of ProIL-?alpha followed by its processing into a 17 kDa mature form by the calcium-dependent, membrane-associated cysteine protease, called calpain (3). Mature IL-1alpha is released into the extracellular compartment while the IL-1alpha N-terminal prodomain (1-115 amino acids) is located in the nucleus (1). The proinflammatory properties of IL-1alpha may be relevant to the development of atherosclerotic vascular disease. IL-1alpha?also has powerful effects on adaptive immunity by enhancing expansion and survival of T cells, differentiation of T helper 17 (Th17) cells and effector T cell proliferation in the presence of regulatory T cells (2). IL-1alpha is not commonly found in the circulation or in body fluids, except if the cytokine might be released from dying cells (4) or secreted upon inflammasome activation (5). Increased IL-1alphaactivity is a hallmark of many chronic inflammatory conditions, including rheumatoid arthritis, gout, diabetes, atherosclerosis and psoriasis (1).
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse IL-1alpha (IL-1alpha) in cell culture supernatants, serum and plasma. A monoclonal antibody specific for IL-1alphahas been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, IL-1alphais recognized by the addition of a biotinylated monoclonal antibody specific for IL-1alpha?(DET). After removal of excess biotinylated antibody, streptavidin-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of IL-1alpha?in the samples!!Background/Introduction: The IL-1 family consists of two pro-inflammatory cytokines IL-1alpha, IL-1beta and the anti-inflammatory IL-1 receptor antagonist (IL-1Ra or IL-1RN). The two IL-1 isoforms (alpha and beta) are encoded by distinct genes but are structurally related and bind to the same receptors that exists in two major forms, Type I (IL-1R1) and Type II (IL-1R2) (1). The major biological roles of IL-1 are described as endogenous pyrogen, leukocyte endogenous mediator, lymphocyte, beta-cell, osteoclast, epidermal cell derived thymocyte activating factor, hematopoietin-1 and mononuclear cell factor (1). IL-1alpha plays a central role in cell growth, tissue repair and chronic inflammatory diseases (1). IL-1alpha is a unique member in the cytokine family which is synthesized as a 33 kDa precursor protein, ProIL-1alpha (1). The mature form p17 results by removal of N-terminal amino acids from ProIL-1alpha. Recently, it has been shown that the mature form p17 of IL-1alpha is the active cytokine. The ProIL-1alpha is poorly active due to its binding to the inhibitory intracellular IL-1R2 protein. Upon inflammasome activation, caspase-1 specifically cleaves IL-1R2, causing release of ProIL-?alpha followed by its processing into a 17 kDa mature form by the calcium-dependent, membrane-associated cysteine protease, called calpain (3). Mature IL-1alpha is released into the extracellular compartment while the IL-1alpha N-terminal prodomain (1-115 amino acids) is located in the nucleus (1). The proinflammatory properties of IL-1alpha may be relevant to the development of atherosclerotic vascular disease. IL-1alpha?also has powerful effects on adaptive immunity by enhancing expansion and survival of T cells, differentiation of T helper 17 (Th17) cells and effector T cell proliferation in the presence of regulatory T cells (2). IL-1alpha is not commonly found in the circulation or in body fluids, except if the cytokine might be released from dying cells (4) or secreted upon inflammasome activation (5). Increased IL-1alphaactivity is a hallmark of many chronic inflammatory conditions, including rheumatoid arthritis, gout, diabetes, atherosclerosis and psoriasis (1).
Product Categories/Family for IL-10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10
Similar Products
Product Notes
The IL-10 (Catalog #AAA495288) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL-10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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