Typical Testing Data/Standard Curve (for reference only)
Progranulin ELISA Kit | granulin elisa kit
Progranulin (Rat) ELISA Kit
Synonyms
Progranulin; Progranulin (Rat) ELISA Kit; granulin elisa kit
Specificity
This ELISA is specific for the measurement of natural and recombinant mouse progranulin. It does not cross-react with mouse RBP4, mouse adiponectin, mouse Nampt, mouse leptin, mouse RELM-beta, mouse IL-33, mouse IDO, mouse resistin, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, human vaspin, human adiponectin, human progranulin, rat Nampt.
Sequence Length
206
Samples
Serum And Cell Culture Supernatant
Assay Type
Sandwich
Detection Range
0.125 ng/ml-8 ng/ml
Sensitivity
60 pg/ml
Intra-assay Precision
Six samples of known concentrations of mouse progranulin were assayed in replicates 8 times to test precision within an assay
Inter-assay Precision
Six samples of known concentrations of mouse progranulin were assayed in 8 separate assays to test precision between assays.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for granulin elisa kit
Intended Uses: The Progranulin mouse ELISA kit is to be used for the measurement of mouse progranulin in serum and cell culture supernatant. This ELISA kit is for research use only.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples!!Background/Introduction: The granulins are a family of cysteine-rich polypeptides, some of which have growth modulatory activity. The widespread occurrence of granulin mRNA in cells from the hematopoietic system and in epithelia implies important functions in these tissues. All 4 known human granulin-like peptides are encoded in a single precursor, progranulin, which has a highly conserved 12-cysteine backbone defining a consensus sequence that is repeated 7 times (1). Progranulin is a 593-amino acid glycoprotein, the mRNA of which is expressed in many epithelial cells both in vitro and in vivo. He and Bateman demonstrated that overexpression of the progranulin gene in SW-13 adrenal carcinoma cells and MDCK nontransformed renal epithelia resulted in transfection-specific secretion of progranulin, acquired clonogenicity in semisolid agar, and increased mitosis in monolayer culture, whereas diminution of progranulin gene expression impaired growth of these cells (2). They proposed that the rate of growth of some epithelia is proportional to the level of intrinsic progranulin gene expression, and that elevated progranulin gene expression confers a transformed phenotype on epithelial cells including anchorage independence in vitro and growth as tumors in nude mice. They also found that in murine transcutaneous puncture wounds, progranulin mRNA is expressed in the inflammatory infiltrate and is highly induced in dermal fibroblasts and endothelia following injury. When applied to a cutaneous wound, progranulin increased the accumulation of neutrophils, macrophages, blood vessels, and fibroblasts in the wound. It acted directly on isolated dermal fibroblasts and endothelial cells to promote division, migration, and the formation of capillary-like tubule structures, concluding that progranulin is, therefore, probably a wound-related growth factor (3). Due to its tumor-promoting activity and multiple functions on hematopoietic cells and endotheial cells, progranulin can be a novel marker for oncology and hematology.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples!!Background/Introduction: The granulins are a family of cysteine-rich polypeptides, some of which have growth modulatory activity. The widespread occurrence of granulin mRNA in cells from the hematopoietic system and in epithelia implies important functions in these tissues. All 4 known human granulin-like peptides are encoded in a single precursor, progranulin, which has a highly conserved 12-cysteine backbone defining a consensus sequence that is repeated 7 times (1). Progranulin is a 593-amino acid glycoprotein, the mRNA of which is expressed in many epithelial cells both in vitro and in vivo. He and Bateman demonstrated that overexpression of the progranulin gene in SW-13 adrenal carcinoma cells and MDCK nontransformed renal epithelia resulted in transfection-specific secretion of progranulin, acquired clonogenicity in semisolid agar, and increased mitosis in monolayer culture, whereas diminution of progranulin gene expression impaired growth of these cells (2). They proposed that the rate of growth of some epithelia is proportional to the level of intrinsic progranulin gene expression, and that elevated progranulin gene expression confers a transformed phenotype on epithelial cells including anchorage independence in vitro and growth as tumors in nude mice. They also found that in murine transcutaneous puncture wounds, progranulin mRNA is expressed in the inflammatory infiltrate and is highly induced in dermal fibroblasts and endothelia following injury. When applied to a cutaneous wound, progranulin increased the accumulation of neutrophils, macrophages, blood vessels, and fibroblasts in the wound. It acted directly on isolated dermal fibroblasts and endothelial cells to promote division, migration, and the formation of capillary-like tubule structures, concluding that progranulin is, therefore, probably a wound-related growth factor (3). Due to its tumor-promoting activity and multiple functions on hematopoietic cells and endotheial cells, progranulin can be a novel marker for oncology and hematology.
Product Categories/Family for granulin elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
progranulin
NCBI Official Symbol
granulin
NCBI Protein Information
progranulin
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Product Notes
The granulin (Catalog #AAA495257) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Progranulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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