Human Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) RTU ELISA Kit | LFA1a rtu elisa kit

Human Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) ELISA Kit

Cat. Num. AAA4500747

• Gene Names: ITGAL; CD11A; LFA-1; LFA1A
• Reactivity: Human
• Synonyms: Lymphocyte Function Associated Antigen 1 Alpha (LFA1a); Human Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) ELISA Kit; CD11a; ITG-AL; LFA-1; LFA1-A; ITGAL; Integrin; Alpha L; CD11 antigen-like family member A; Leukocyte function-associated molecule 1 alpha chain; LFA1a rtu elisa kit

• Reactivity: Human
• Sequence Length: 1086

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LFA1a rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LFA1a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LFA1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LFA1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LFA1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 167466217
• NCBI GeneID: 3683
• NCBI Accession #: NP_001107852.1
• NCBI GenBank Nucleotide #: NM_001114380.1
• UniProt Accession #: P20701; O43746; Q45H73; Q96HB1; Q9UBC8
• Molecular Weight: 119,224 Da
• NCBI Official Full Name: integrin alpha-L isoform b
• NCBI Official Synonym Full Names: integrin subunit alpha L
• NCBI Official Symbol: ITGAL
• NCBI Official Synonym Symbols: CD11A; LFA-1; LFA1A
• NCBI Protein Information: integrin alpha-L
• UniProt Protein Name: Integrin alpha-L
• UniProt Gene Name: ITGAL
• UniProt Synonym Gene Names: CD11A; LFA-1A

NCBI Description

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin alpha-L/beta-2 is also a receptor for F11R (PubMed:11812992, PubMed:15528364). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).