Typical Testing Data/Standard Curve (for reference only)
RBP4 elisa kit
RBP4 (Mouse/Rat) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human RBP4 in biological fluids. A monoclonal antibody specific for RBP4 has been pre-coated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, RBP4 is recognized by the addition of a purified polyclonal antibody specific for RBP4 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using 3,3',5,5'-tetramethyl-benzidine (TMB) as substrate. The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of human RBP4 in the samples!!Background/Introduction: Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues (1). In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue (2). Yang et al. noted that adipocyte-specific deletion of Gluts led to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improved insulin resistance (3). This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. Graham et al. showed that serum RBP4 levels correlated with the magnitude of insulin resistance in subjects with obesity, impaired glucose tolerance, or type 2 diabetes (4). Cho et al. recently showed that plasma RBP4 concentrations are elevated in human subjects with impaired glucose tolerance and type 2 diabetes (5). Thus, measurement of serum or plasma RBP4 is a useful means for understanding of metabolic disorders.
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
Uniprot Description
RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q23.33
Cellular Component: cytosol; extracellular region; extracellular space; protein complex
Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding
Biological Process: cardiac muscle development; embryonic organ morphogenesis; embryonic retina morphogenesis in camera-type eye; embryonic skeletal development; eye development; fat-soluble vitamin metabolic process; female genitalia morphogenesis; gluconeogenesis; glucose homeostasis; heart development; lung development; maintenance of gastrointestinal epithelium; negative regulation of cardiac muscle cell proliferation; phototransduction, visible light; positive regulation of immunoglobulin secretion; positive regulation of insulin secretion; response to ethanol; response to retinoic acid; retinoid metabolic process; retinol metabolic process; urinary bladder development; uterus development; vagina development; visual perception; vitamin metabolic process
Disease: Microphthalmia, Isolated, With Coloboma 10; Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome
Research Articles on RBP4
Similar Products
Product Notes
The RBP4 rbp4 (Catalog #AAA495306) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RBP4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
