Mouse Gasdermin D ELISA Kit

Gasdermin D (Mouse) ELISA

Cat. Num. AAA490395

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Gasdermin D; Gasdermin D (Mouse) ELISA; GASDERMIN D (MOUSE); Gasdermin D elisa kit

• Reactivity: Mouse
• Specificity: This ELISA is specific for the measurement of natural and recombinant mouse Gasdermin D (full-length and C-terminus cleaved fragment). It does not detect human Gasdermin D. Gasdermin D is tested from supernatants of Bone Marrow-Derived Macrophages cells (BMDMs) transfected with LPS from different knockout mice strains (see figure 1). Only the supernatants from WT and NLRP3-/- strains contain the protein Gasdermin D. Gasdermin D is also tested from cell extracts (lysed with a Triton X-100 buffer) of Bone Marrow-Derived Macrophages cells from WT and Gasdermin D knockout mice strains (Figure 2). Both figures confirm the specificity of the Gasdermin (mouse) ELISA Kit.

• Applicable Applications for Gasdermin D elisa kit: ELISA (EIA)
• Samples: Cell culture supernatants and cell extracts
• Detection Range: 15.625 pg/ml - 1000 pg/ml
• Sensitivity: 14 pg/ml
• Intra-assay Precision: Four samples of known concentrations of mouse Gasdermin D were assayed in replicates 8 times to test precision within an assay.
• Inter-assay Precision: Four samples of known concentrations of mouse Gasdermin D were assayed in 5 separate assays to test precision between assays.
• Preparation and Storage: Store at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Gasdermin D elisa kit

Intended Uses: The Gasdermin D (mouse) ELISA Kit is to be used for the quantitative determination of mouse Gasdermin D in cell culture supernatants and cell extracts. This ELISA Kit is for research use only.

Background/Introduction: Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC /Pycard) and a protease (procaspase-1) (1). An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs), or perturbations in cytoplasmic homeostasis (term 'homeostasis-altering molecular processes' (HAMPs))(2). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukins 1beta and 18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, cleave the newly discovered intracellular protein Gasdermin D (3,4). The Gasdermin family members contain N-terminal domains that are capable of forming membrane pores, whereas the C-terminal domains of Gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or 11 cleavage of Gasdermin D is required for regulation of pyroptosis: upon caspase 1/11 cleavage of the Gasdermin N- and C-domain linker, the cleaved N-terminal fragment of Gasdermin D oligomerizes and forms pores on the host cell membrane (5), leading to a cell death called pyroptosis and further activation of inflammasomes by triggering K+ efflux (6). Gasdermin D forming pores regulate the non-conventional secretion of cytokines such as IL-1beta, in response to cytosolic LPS and other activators of the inflammasome (7). Neutrophil extrusion of neutrophil extracellular traps (NETs) and concomitant cell death (NETosis), a particular neutrophil defense against pathogens, are dependent on Gasdermin D (8). Gasdermin D-mediated pyroptosis is regulated at the level of lipid peroxidation (9) and seems to be a key effector in the LPS-induced lethal sepsis (10).