GPX3 elisa kit

GPX3 ELISA Kit

Cat. Num. AAA495226

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Synonyms: GPX3; GPX3 ELISA Kit; Glutathione peroxidase 3; GPX3 elisa kit

• Specificity: This ELISA is specific for the measurement of natural and recombinant human GPX1. It does not cross-react with human GPX2, human GPX3, human GPX4, human IL-33, human ST2, human adiponectin, human RBP4, human Nampt, human vaspin, human progranulin, human resistin, human clusterin, human ANGPTL3, human CTRP5, human ACE2, human leptin, mouse GPX3, mouse Nampt, rat Nampt.
• Sequence Length: 226

• Samples: Cell Lysates Or Cell-Based Assays (Screening)
• Assay Type: Sandwich
• Detection Range: 0.063 ng/ml-4 ng/ml
• Sensitivity: 45 pg/ml
• Intra-assay Precision: Six samples of known concentrations of human GPX1 were assayed in replicates 5 times to test precision within an assay
• Inter-assay Precision: Six samples of known concentrations of human GPX1 were assayed in 5 separate assays to test precision between assays.
• Preparation and Storage: 2 - 8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPX3 elisa kit

Intended Uses: The GPX1 human (Intracellular) ELISA is to be used for the measurement of human GPX1 in cell lysates or cell-based assays (screening). This ELISA kit is for research use only.

Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human GPX1 in cells. A polyclonal antibody specific for GPX1 has been pre-coated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, GPX1 is recognized by the addition of a purified polyclonal antibody specific for GPX1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of GPX1 in the samples!!Background/Introduction: Glutathione peroxidase1 (GPX1) functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon (1-2). It has been (De Haan et al. (1998) demonstrated a role for GPX1 in protection against oxidative stress by showing that Gpx1-/-mice are highly sensitive to an oxidant compared to wildtype controls (3). The oxidant transcriptionally upregulated Gpx1 in normal cells, reinforcing a role for GPX1 in protection against its toxicity. Cortical neurons from Gpx1-/-mice are more susceptible to peroxide whereas the wildtype controls were unaffected. This data suggest that GPX1 in protection against some oxidative stressors and in protection of neurons against peroxide. Chronic hyperglycemia causes oxidative stress, which contributes to damage in various tissues and cells, including pancreatic b-cells. The expression levels of antioxidant enzymes in the islet are low compared with other tissues, rendering the b-cell more susceptible to damage caused by hyperglycemia. A direct evidence for involvement of GPX1 in beta cell function was given by creating a transgenic mouse bearing b-cell-specific expression of GPX1 (4). The biological effectiveness of the overexpressed GPx-1 transgene was documented when b-cells of transgenic mice were protected from streptozotocin. When breeded with db/db mice hyperglycemia in db/db-GPx(+) mice was ameliorated compared with db/db-GPx(-). b-cell volume and insulin granulation and immunostaining were greater in db/db-GPx(+) animals compared with db/db-GPx(-) animals, demonstrating that GPX1 overexpression protects b-cell against deterioration during hyperglycemia.

Product Categories/Family for GPX3 elisa kit

Research Assays; Assay Categories

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075.2
• NCBI GenBank Nucleotide #: NM_002084.3
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase 3
• NCBI Official Synonym Full Names: glutathione peroxidase 3
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Secreted; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular region; extracellular space

Molecular Function: glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding

Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide; response to reactive oxygen species