Human CCL7/MCP-3 ELISA Kit | CCL7 elisa kit

Human CCL7/MCP-3 Quick ELISA Kit

Cat. Num. AAA1753914

• Reactivity: Human
• Synonyms: CCL7/MCP-3; Human CCL7/MCP-3 Quick ELISA Kit; CCL7/MCP-3/MARC|C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3); CCL7 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CCL7

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <1pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.019|0.081|0.135|0.236|0.416|0.750|1.425|2.307
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|35|175|512|; Standard deviation|2.77|12.78|28.16|br>CV(%)|7.9%|7.3%|5.5%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|41|166|506; Standard deviation|2.83|13.11|30.87; CV(%)|6.9%|7.9%|6.1%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 35|31|36|41|36|3.32|9.3%; Sample 2: 175|166|170|169|170|2.25|1.5%; Sample 3: 512|526|443|429|478|39.63|8.3%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E Coli; Immunogen sequence: Q24-L99
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human CCL7/MCP-3 with <1pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human CCL7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CCL7 with immunogen: Expression system for standard: E Coli; Immunogen sequence: Q24-L99.; ; To measure Human CCL7, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CCL7 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL7 in the sample.
• Assay Procedure Step 1: 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for CCL7 elisa kit

Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11. 2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Product Categories/Family for CCL7 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 313708
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: monocyte chemotactic protein-3
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; monocyte chemotaxis; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; cytoskeleton organization and biogenesis; immune response; inflammatory response; chemotaxis; signal transduction; positive regulation of cell migration