Mouse MMP-2 ELISA Kit | Mmp2 elisa kit

Mouse MMP-2 Quick ELISA Kit

Cat. Num. AAA1753916

• Gene Names: Mmp2; GelA; Clg4a; MMP-2
• Reactivity: Mouse
• Synonyms: MMP-2; Mouse MMP-2 Quick ELISA Kit; MMP-2|72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3. 4. 24; EC 3. 4. 24. 24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A; 72kD gelatinase; 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A; 72kDa type IV collagenase); Mmp2 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Mmp2

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum and plasma (heparin)
• Detection Range: 625pg/ml-40,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|625|1250|2500|5000|10000|20000|40000; O.D.|0.029|0.115|0.173|0.310|0.537|0.976|1.555|2.153
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|1401|2986|17423|; Standard deviation|60.24|134.37|731.77|; CV(%)|4.3%|4.5%|4.2%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|1521|3364|17532; Standard deviation88.22|161.47|806.47; CV(%)|5.8%|4.8%|4.6%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 1401|1416|1369|1426|1403|44.90|3.2%; Sample 2: 2986|3531|3163|3154|3209|144.38|4.5%; Sample 3: 17423|15436|16428|16654|16485|560.5|3.4%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A30-C662
• Anticoagulant: heparin
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Mouse MMP-2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Mouse Mmp2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Mmp2 in cell culture supernatants, serum and plasma (heparin). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Mouse Mmp2 with immunogen: Expression system for standard: NS0; Immunogen sequence: A30-C662.; ; To measure Mouse Mmp2, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Mmp2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Mmp2 in the sample.
• Assay Procedure Step 1: 40000pg/ml, 20000pg/ml,10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for Mmp2 elisa kit

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Product Categories/Family for Mmp2 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 461766
• NCBI GeneID: 17390
• UniProt Accession #: P33434
• Molecular Weight: 65,883 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Official Synonym Symbols: GelA; Clg4a; MMP-2
• NCBI Protein Information: 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_MOUSE

Uniprot Description

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.

Protein type: Protease; Secreted; Apoptosis; Cell development/differentiation; EC 3.4.24.24; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; sarcomere; membrane; mitochondrion; cytoplasm; plasma membrane; extracellular region; nucleus

Molecular Function: peptidase activity; zinc ion binding; hydrolase activity; metallopeptidase activity; metal ion binding; metalloendopeptidase activity; fibronectin binding

Biological Process: response to drug; cell migration; intramembranous ossification; positive regulation of apoptosis; tissue remodeling; negative regulation of cell adhesion; proteolysis; axon regeneration in the peripheral nervous system; blood vessel maturation; negative regulation of vasoconstriction; collagen catabolic process; response to hydrogen peroxide; ovarian follicle development; response to estrogen stimulus; skeletal morphogenesis; response to mechanical stimulus; positive regulation of innate immune response; response to hypoxia; response to oxidative stress; angiogenesis; embryo implantation; positive regulation of cell migration