Mouse CCL7/MCP3 ELISA Kit | CCL7 elisa kit

Mouse CCL7/MCP3 PicoKine ELISA Kit

Cat. Num. AAA177240

• Gene Names: Ccl7; fic; marc; mcp3; MCP-3; Scya7
• Reactivity: Mouse
• Synonyms: CCL7/MCP3; Mouse CCL7/MCP3 PicoKine ELISA Kit; C-C motif chemokine 7; chemokine (C-C motif) ligand 7; Ccl7; CCL7_HUMAN; Chemokine CC Motif Ligand 7; Chemotactic protein 3; FIC; Intercrine/chemokine MARC; MARC; MCP 3; MCP-3; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RANTES/sis homolog; RP23-350G1.4; SCYA6; SCYA7; Small inducible cytokine A7; Small-inducible cytokine A7; CCL7 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CCL7
• Sequence Length: 97

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CCL7/MCP3 PicoKine ELISA Kit standard curve )

Related Product Information for CCL7 elisa kit

Principle of the assay: mouse CCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL7 has been precoated onto 96-well plates. Standards(E.coli, Q24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL7 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

NCBI and Uniprot Product Information

• NCBI GI #: 417192
• NCBI GeneID: 20306
• UniProt Accession #: Q03366
• Molecular Weight: 10,999 Da
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 7
• NCBI Official Symbol: Ccl7
• NCBI Official Synonym Symbols: fic; marc; mcp3; MCP-3; Scya7
• NCBI Protein Information: C-C motif chemokine 7; RANTES/sis homolog; intercrine/chemokine MARC; small inducible cytokine A7; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl7
• UniProt Synonym Gene Names: Fic; Mcp3; Scya7; MCP-3
• UniProt Entry Name: CCL7_MOUSE

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; chemokine activity; CCR2 chemokine receptor binding; cytokine activity

Biological Process: immune response; inflammatory response; chemotaxis