Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Monocyte chemotactic protein 3 (MCP-3) Standard Curve.)
Mouse Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit | MCP-3 elisa kit
Mouse Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit
Gene Names
Ccl7; fic; marc; mcp3; MCP-3; Scya7
Reactivity
Mouse
Synonyms
Monocyte Chemotactic Protein 3 (MCP-3); Mouse Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit; CCL7; FIC; MARC; MCP-3; MCP3; MGC138463; MGC138465; NC28; SCYA6; SCYA7; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3); MCP-3 elisa kit
Reactivity
Mouse
Specificity
Mouse Monocyte chemotactic protein 3 (MCP-3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CCL7. No significant cross-reactivity or interference between Mouse CCL7 and analogues was observed.
Sequence Length
97
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Monocyte chemotactic protein 3 (MCP-3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Monocyte chemotactic protein 3 (MCP-3) Standard Curve.)
Related Product Information for MCP-3 elisa kit
Description: This Mouse Monocyte chemotactic protein 3 (MCP-3) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL7 in samples. An antibody specific for CCL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines.The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. CCL7 is one of the most pluripotent chemokines, produced by certain tumor cell lines and by macrophages and acting on multiple cell types including monocytes, lymphocytes, eosinophils, basophils, dendritic cells, and natural killer cells.
Background: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines.The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. CCL7 is one of the most pluripotent chemokines, produced by certain tumor cell lines and by macrophages and acting on multiple cell types including monocytes, lymphocytes, eosinophils, basophils, dendritic cells, and natural killer cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C motif chemokine 7
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 7
NCBI Official Symbol
Ccl7
NCBI Official Synonym Symbols
fic; marc; mcp3; MCP-3; Scya7
NCBI Protein Information
C-C motif chemokine 7
UniProt Protein Name
C-C motif chemokine 7
UniProt Gene Name
Ccl7
UniProt Synonym Gene Names
Fic; Mcp3; Scya7; MCP-3
UniProt Entry Name
CCL7_MOUSE
Uniprot Description
CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; cytokine activity
Biological Process: immune response; chemotaxis; inflammatory response
Research Articles on MCP-3
Similar Products
Product Notes
The Mouse MCP-3 ccl7 (Catalog #AAA9718154) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718154 ELISA Kit recognizes Mouse MCP-3. It is sometimes possible for the material contained within the vial of "Monocyte Chemotactic Protein 3 (MCP-3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
