Rat CCL7 ELISA Kit | CCL7 elisa kit

Rat CCL7 ELISA Kit

Cat. Num. AAA8244676

• Reactivity: Rat
• Applications: ELISA
• Synonyms: CCL7; Rat CCL7 ELISA Kit; MCP3; SCYA6; SCYA7; C-C motif chemokine 7; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; NC28; Small-inducible cytokine A7; CCL7 elisa kit

• Reactivity: Rat
• Specificity: The Rat CCL7 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CCL7 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 97

• Applicable Applications for CCL7 elisa kit: Sandwich ELISA (EIA)
• Samples: Serum, plasma
• Sensitivity: Typically less than 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Standard Curve/Testing Data

Related Product Information for CCL7 elisa kit

Principle of the Assay: The Rat CCL7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CCL7 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

NCBI and Uniprot Product Information

• NCBI GI #: 9087193
• NCBI GeneID: 287561
• UniProt Accession #: Q9QXY8
• Molecular Weight: 10,906 Da
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 7
• NCBI Official Symbol: Ccl7
• NCBI Protein Information: C-C motif chemokine 7
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl7
• UniProt Synonym Gene Names: Mcp3; Scya7; MCP-3
• UniProt Entry Name: CCL7_RAT

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space

Molecular Function: CCR chemokine receptor binding; CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity; heparin binding

Biological Process: cytoskeleton organization and biogenesis; eosinophil chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of inflammatory response; regulation of cell shape; response to gamma radiation