Rat MMP-2 ELISA Kit | Mmp2 elisa kit

Rat MMP-2 Quick ELISA Kit

Cat. Num. AAA1753924

• Reactivity: Rat
• Synonyms: MMP-2; Rat MMP-2 Quick ELISA Kit; MMP-2|72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3. 4. 24; EC 3. 4. 24. 24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A; 72kD gelatinase; 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A; 72kDa type IV collagenase); Mmp2 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Mmp2

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum and plasma (heparin)
• Detection Range: 625pg/ml-40,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|625|1250|2500|5000|10000|20000|40000; O.D.|0.029|0.115|0.173|0.310|0.537|0.976|1.555|2.153
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|2001|4516|23614|; Standard deviation|176.09|320.64|1157.09|; CV(%)|8.8%|7.1%|4.9%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|1093|4543|21263; Standard deviation|86.35|367.98|1318.31; CV(%)|7.9%|8.1%|6.2%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 2001|1093|986|1110|1298|63.58|4.9%; Sample 2: 4516|3756|4531|4695|4375|253.72|5.8%; Sample 3: 23614|23697|22457|26546|24079|1396.55|5.8%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A30-C662
• Anticoagulant: heparin
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Rat MMP-2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Rat Mmp2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Rat Mmp2 with immunogen: Expression system for standard: NS0; Immunogen sequence: A30-C662.; ; To measure Rat Mmp2, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Mmp2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Mmp2 in the sample.
• Assay Procedure Step 1: 40000pg/ml, 20000pg/ml,10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for Mmp2 elisa kit

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. 1 Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. 2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. 3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Product Categories/Family for Mmp2 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 146345459
• NCBI GeneID: 81686
• UniProt Accession #: P33436; P97581; Q6GMM9
• Molecular Weight: 74,149 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Protein Information: 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase 2; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_RAT

NCBI Description

plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]

Uniprot Description

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().