Typical Testing Data/Standard Curve (for reference only)
Human MCP-3 ELISA Kit | MCP-3 elisa kit
MCP-3 Human ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
MCP-3; MCP-3 Human ELISA Kit; Human MCP-3; MCP-3 elisa kit
Reactivity
Human
Applicable Applications for MCP-3 elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of MCP-3 in human serum, plasma, urine, cell culture supernatant or tissue samples.
Assay Type
Sandwich
Detection Range
15 pg/ml-800 pg/ml
Preparation and Storage
Store at 2-8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MCP-3 elisa kit
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MCP-3 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MCP-3 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-3 can be calculated.
Background: Monocyte-specific chemokine 3 (MCP3), also called chemokine (C-C motif) ligand 7 (CCL7), is a small cytokine known as a chemokine that belongs to the C-C chemokine family. It is produced by certain tumor cell lines and by macrophages. This chemokine is located on chromosome 17 in humans, in a large cluster containing many other CC chemokines and is most closely related to CCL2 (previously called MCP1).It was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation.
Background: Monocyte-specific chemokine 3 (MCP3), also called chemokine (C-C motif) ligand 7 (CCL7), is a small cytokine known as a chemokine that belongs to the C-C chemokine family. It is produced by certain tumor cell lines and by macrophages. This chemokine is located on chromosome 17 in humans, in a large cluster containing many other CC chemokines and is most closely related to CCL2 (previously called MCP1).It was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation.
NCBI and Uniprot Product Information
Uniprot Description
Function: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Subunit structure: Monomer. Ref.8
Subcellular location: Secreted.
Post-translational modification: O-glycosylated.
Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
Sequence caution: The sequence CAA50405.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA50406.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA51055.1 differs from that shown. Reason: Erroneous initiation.
Similar Products
Product Notes
The Human MCP-3 ccl7 (Catalog #AAA355290) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355290 ELISA Kit recognizes Human MCP-3. AAA Biotech's MCP-3 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of MCP-3 in human serum, plasma, urine, cell culture supernatant or tissue samples. Researchers should empirically determine the suitability of the MCP-3 ccl7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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