Human TWEAK CLIA Kit | TWEAK clia kit

TWEAK (Human) LumiAb ELISA Kit

Cat. Num. AAA9510824

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK; TNLG4A
• Reactivity: Human
• Synonyms: TWEAK; TWEAK (Human) LumiAb ELISA Kit; Human TWEAK; Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; APO3L; DR3LG; TWEAK clia kit

• Reactivity: Human
• Specificity: The LumiAb Human TPO ELISA is capable of recognizing both recombinant and naturally produced Human TPO proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 780

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TWEAK clia kit

Principle of the Assay: The LumiAb Human TPO Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human TPO concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human TPO while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human TPO, also known as Thyroid Peroxidase, is a 933 amino acid enzyme encoded by the TPO gene located at locus 2p25 on chromosome 2. After initial synthesis of the peptide, the protein is proteolytically cleaved into its 14 residue signal peptide and mature 919 residue TPO protein. TPO is essentially a membrane-bound glycoprotein that acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination and coupling of hormonogenic tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones: thyroxine (T4) and triiodothyronine (T3). TPO is stimulated by TSH, which upregulates gene expression and is inhibited by the thioamide drugs such as propylthiouracil and methimazole. Mutations in this gene are often associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full length nature of some variants has not been determined.

Product Categories/Family for TWEAK clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 74958325
• NCBI GeneID: 8742
• UniProt Accession #: O44008; O43508
• NCBI Official Full Name: Valosin-containing protein homolog
• NCBI Official Synonym Full Names: TNF superfamily member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK; TNLG4A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]