Human PAI-1 CLIA Kit | PAI-1 clia kit

PAI-1 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510809

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Human
• Synonyms: PAI-1; PAI-1 (Human) LumiAb ELISA Kit; Human PAI-1; Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; Serpin E1; PLANH1; PAI-1 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human PLGF-1 ELISA is capable of recognizing both recombinant and naturally produced Human PLGF-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 402

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAI-1 clia kit

Principle of the Assay: The LumiAb Human P1GF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human P1GF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human P1GF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human P1GF-1, also known as Placenta Growth Factor, is a 221 amino acid growth factor protein encoded by the PGF gene located at locus 14q24.3 on chromosome 14. After initial synthesis, the 18 residue signal sequence is removed, allowing for the proper folding and maturation of the 203 residue PLGF-1 protein. PLGF-1 is essentially a growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1 while isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. The subunit structure is known to be an antiparallel homodimer connected via disulfide linkages, but also can be found as a heterodimer with VEGFA/VEGF. Meanwhile, the PLGF-3 isoform can be found as both as a homodimer and as a monomer. While the three isoforms are present in most placental tissues, PLGF-2 is specific to early (8 week) placenta and only PLGF-1 is found in the colon and mammary carcinomas.

Product Categories/Family for PAI-1 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• UniProt Accession #: P05121
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin family E member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; platelet degranulation; negative regulation of fibrinolysis; transforming growth factor beta receptor signaling pathway; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; platelet activation; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; positive regulation of angiogenesis; fibrinolysis; positive regulation of transcription from RNA polymerase II promoter; gene expression; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency