Mouse G-CSF CLIA Kit | G-CSF clia kit

G-CSF (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510828

• Gene Names: Csf3; Csfg; G-CSF; MGI-IG
• Reactivity: Mouse
• Synonyms: G-CSF; G-CSF (Murine) LumiAb ELISA Kit; Murine G-CSF; Granulocyte colony-stimulating factor; Csfg; G-CSF clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine Eotaxin ELISA is capable of recognizing both recombinant and naturally produced Murine Eotaxin proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 208

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for G-CSF clia kit

Principle of the Assay: The LumiAb Murine Eotaxin Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine Eotaxin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine Eotaxin while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine Eotaxin, also known as C-C Chemokine 11, is a 97 amino acid cytokine protein encoded by the Ccl11 gene located on chromosome 11. After initial synthesis and translocation, the 23 residue signal sequence is cleaved from the N-terminal region, allowing the 74 residue Eotaxin peptide to fold properly into its mature form. In response to the presence of allergens, Eotaxin, an intercrine beta (chemokine C-C) family member, directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Furthermore, it is expressed constitutively in the thymus, while expression in the lungs (type I alveolar epithelial cells), intestines, heart, spleen and kidneys is inducible by IFN-gamma and lipopolysaccharides (LPS).

Product Categories/Family for G-CSF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6753536
• NCBI GeneID: 12985
• NCBI Accession #: NP_034101.1
• NCBI GenBank Nucleotide #: NM_009971.1
• UniProt Accession #: P09920
• NCBI Official Full Name: granulocyte colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: Csf3
• NCBI Official Synonym Symbols: Csfg; G-CSF; MGI-IG
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: Csf3
• UniProt Synonym Gene Names: Csfg; G-CSF
• UniProt Entry Name: CSF3_MOUSE

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: enzyme binding; growth factor activity; cytokine activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein binding; positive regulation of actin filament polymerization; positive regulation of myeloid cell differentiation; granulocyte differentiation; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation