Rat EGF CLIA Kit | EGF clia kit

EGF (Rat) LumiAb ELISA Kit

Cat. Num. AAA9510857

• Reactivity: Rat
• Synonyms: EGF; EGF (Rat) LumiAb ELISA Kit; Rat EGF; Epidermal growth factor; EGF clia kit

• Reactivity: Rat
• Specificity: The LumiAb Murine VEGF ELISA is capable of recognizing both recombinant and naturally produced Murine VEGF proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 1133

• Assay Type: Quantitative Sandwich
• Sensitivity: 6 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EGF clia kit

Principle of the Assay: The LumiAb Murine VEGF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine VEGF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine VEGF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine VEGF or Tumor Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor, is a 214 amino acid cytokine protein expressed from the Vegf gene located on chromosome 17. After initial synthesis and translocation, the 26 residue signal sequence is cleaved, allowing the 188 residue VEGF peptide to fold properly. VEGF is a homodimeric, disulfide linked, growth factor active in angiogenesis, vasculogenesis and endothelial cell growth that functions to induce endothelial cell proliferation, promote cell migration, inhibit apoptosis and induce permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparin sulfate and heparin. Moreover, it may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. In developing embryos, VEGF is mainly expressed in the choroid plexus, paraventricular neuroepithelium, and placenta and kidney glomeruli. It is also found in bronchial epithelium, adrenal gland and in seminiferous tubules of the testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults. Levels increase during pregnancy (maximum 5.5-fold at 5 days) and a more marked increase occurs during lactation (maximal 9.7-fold at 7 days) but decrease progressively during the phase of involution.

Product Categories/Family for EGF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6978797
• NCBI GeneID: 25313
• NCBI Accession #: NP_036974.1
• NCBI GenBank Nucleotide #: NM_012842.1
• UniProt Accession #: P07522
• NCBI Official Full Name: pro-epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_RAT

NCBI Description

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Hormone; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; STAT protein nuclear translocation; positive regulation of DNA binding; positive regulation of granule cell precursor proliferation